Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11894] to GNAI3
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-GNAI3 antibody [EPR11894]
See all GNAI3 primary antibodies
DescriptionRabbit monoclonal [EPR11894] to GNAI3
SpecificityThe peptide immunogen shares a 92.9% homology with GNAI1 and GNAI2, therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Guinea pig, Cow, Xenopus laevis
Synthetic peptide within Human GNAI3 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P08754
- Human fetal brain, fetal kidney, HepG2 and A431 lysates. Immunoprecipitation pellet from Human fetal brain lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab173527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 41 kDa.|
|IP||1/10 - 1/100.|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
Cellular localizationCytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
- Information by UniProt
- 87U6 antibody
- FLJ26559 antibody
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All lanes : Anti-GNAI3 antibody [EPR11894] (ab173527) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human fetal kidney lysates
Lane 3 : HepG2 lysates
Lane 4 : A431 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab173527 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab173527 has been referenced in 1 publication.
- Freeman S et al. Proteostasis is essential during cochlear development for neuron survival and hair cell polarity. EMBO Rep 20:e47097 (2019). PubMed: 31321879