Overview

  • Product name

    Anti-GNAI3 antibody [EPR11894]
    See all GNAI3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11894] to GNAI3
  • Host species

    Rabbit
  • Specificity

    The peptide immunogen shares a 92.9% homology with GNAI1 and GNAI2, therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Guinea pig, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human GNAI3 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P08754

  • Positive control

    • Human fetal brain, fetal kidney, HepG2 and A431 lysates. Immunoprecipitation pellet from Human fetal brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11894
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
    • Sequence similarities

      Belongs to the G-alpha family. G(i/o/t/z) subfamily.
    • Cellular localization

      Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
    • Information by UniProt
    • Database links

    • Alternative names

      • 87U6 antibody
      • FLJ26559 antibody
      • G protein alpha inhibiting 3 antibody
      • G(i) alpha 3 antibody
      • G(i) alpha-3 antibody
      • GNAI3 antibody
      • GNAI3_HUMAN antibody
      • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3 antibody
      • Guanine nucleotide binding protein G(k) alpha subunit antibody
      • Guanine nucleotide-binding protein G(k) subunit alpha antibody
      • OTTHUMP00000013368 antibody
      see all

    Images

    • All lanes : Anti-GNAI3 antibody [EPR11894] (ab173527) at 1/1000 dilution

      Lane 1 : Human fetal brain lysates
      Lane 2 : Human fetal kidney lysates
      Lane 3 : HepG2 lysates
      Lane 4 : A431 lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab173527 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab173527 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Purified protein (BL21 RIL competent cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris Gel)
    Loading amount
    0.5 µg
    Specification
    BL21 RIL competent cells
    Blocking step
    iBind Solution as blocking agent for 10 minute(s) · Concentration: 100%

    Mr. Kevin Ebert

    Verified customer

    Submitted Sep 28 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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