Recombinant
RabMAb

Anti-GNAI3 antibody [EPR11900] (ab176339)

Overview

  • Product name
    Anti-GNAI3 antibody [EPR11900]
    See all GNAI3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11900] to GNAI3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GNAI3 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P08754

  • Positive control
    • Fetal brain, Fetal kidney, and A431 lysates. Paraffin-embedded Human pancreas tissue, paraffin-embedded Human skeletal muscle tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11900
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
    • Sequence similarities
      Belongs to the G-alpha family. G(i/o/t/z) subfamily.
    • Cellular localization
      Cytoplasm. Cell membrane. Cytoplasm > cytoskeleton > centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
    • Information by UniProt
    • Database links
    • Alternative names
      • 87U6 antibody
      • FLJ26559 antibody
      • G protein alpha inhibiting 3 antibody
      • G(i) alpha 3 antibody
      • G(i) alpha-3 antibody
      • GNAI3 antibody
      • GNAI3_HUMAN antibody
      • Guanine nucleotide binding protein (G protein) alpha inhibiting activity polypeptide 3 antibody
      • Guanine nucleotide binding protein G(k) alpha subunit antibody
      • Guanine nucleotide-binding protein G(k) subunit alpha antibody
      • OTTHUMP00000013368 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized A431 cells labeling GNAI3 with ab176339 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling GNAI3 with ab176339 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GNAI3 with ab176339 at 1/50 dilution.

    • All lanes : Anti-GNAI3 antibody [EPR11900] (ab176339) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal kidney lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    References

    ab176339 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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