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  1. Link

    gnao1-antibody-ab154001.pdf

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Anti-GNAO1 antibody (ab154001)

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Western blot - Anti-GNAO1 antibody (ab154001)
  • Western blot - Anti-GNAO1 antibody (ab154001)
  • Immunocytochemistry/ Immunofluorescence - Anti-GNAO1 antibody (ab154001)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (ab154001)

Key features and details

  • Rabbit polyclonal to GNAO1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GNAO1 antibody
    See all GNAO1 primary antibodies
  • Description

    Rabbit polyclonal to GNAO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human GNAO1 aa 104-338.

  • Positive control

    • A549, HeLa, Jurkat, NT2D1 and mouse brain lysates; HeLa cells; Human lung adenocarcinoma tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Adrenergic Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Muscarinic Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • G Proteins
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Jurkat whole cell lysate (ab7899)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.
  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Target information above from: UniProt accession P09471 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281792 Cow
    • Entrez Gene: 2775 Human
    • Entrez Gene: 14681 Mouse
    • Entrez Gene: 50664 Rat
    • Entrez Gene: 394423 Xenopus laevis
    • Entrez Gene: 397896 Xenopus laevis
    • Entrez Gene: 549749 Xenopus tropicalis
    • Omim: 139311 Human
    • SwissProt: P08239 Cow
    • SwissProt: P09471 Human
    • SwissProt: P18872 Mouse
    • SwissProt: P59215 Rat
    • SwissProt: P10825 Xenopus laevis
    • Unigene: 644524 Human
    • Unigene: 251445 Mouse
    • Unigene: 90161 Rat
    • Unigene: 1200 Xenopus laevis
    see all
  • Alternative names

    • DKFZp686O0962 antibody
    • G alpha o antibody
    • G protein alpha 0 antibody
    • Galphao antibody
    • GNAO 1 antibody
    • GNAO antibody
    • GNAO_HUMAN antibody
    • GNAO1 antibody
    • Go alpha antibody
    • Guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O antibody
    • Guanine nucleotide binding protein alpha activating polypeptide antibody
    • Guanine nucleotide binding protein G(o) subunit alpha antibody
    • Guanine nucleotide-binding protein G(o) subunit alpha antibody
    see all

Images

  • Western blot - Anti-GNAO1 antibody (ab154001)
    Western blot - Anti-GNAO1 antibody (ab154001)
    Anti-GNAO1 antibody (ab154001) at 1/10000 dilution + Mouse brain whole cell
    at 5 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Western blot - Anti-GNAO1 antibody (ab154001)
    Western blot - Anti-GNAO1 antibody (ab154001)
    All lanes : Anti-GNAO1 antibody (ab154001) at 1/1000 dilution

    Lane 1 : HeLa whole cell
    lysate
    Lane 2 : NT2D1 whole cell
    lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-GNAO1 antibody (ab154001)
    Immunocytochemistry/ Immunofluorescence - Anti-GNAO1 antibody (ab154001)

    Immunofluorescent analysis of methanol-fixed HeLa cells labeling GNAO1 with ab154001 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (ab154001)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (ab154001)

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling GNAO1 with ab154001 at 1/500 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab154001? Please let us know so that we can cite the reference in this datasheet.

ab154001 has not yet been referenced specifically in any publications.

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