Anti-GNAO1 antibody (ab154001)
Key features and details
- Rabbit polyclonal to GNAO1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GNAO1 antibody
See all GNAO1 primary antibodies -
Description
Rabbit polyclonal to GNAO1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Monkey, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to Human GNAO1 aa 104-338.
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Positive control
- A549, HeLa, Jurkat, NT2D1 and mouse brain lysates; HeLa cells; Human lung adenocarcinoma tissue.
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General notes
This product was previously labelled as G0 Protein alpha
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. - Information by UniProt
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Database links
- Entrez Gene: 281792 Cow
- Entrez Gene: 2775 Human
- Entrez Gene: 14681 Mouse
- Entrez Gene: 50664 Rat
- Entrez Gene: 394423 Xenopus laevis
- Entrez Gene: 397896 Xenopus laevis
- Entrez Gene: 549749 Xenopus tropicalis
- Omim: 139311 Human
see all -
Alternative names
- DKFZp686O0962 antibody
- G alpha o antibody
- G protein alpha 0 antibody
see all
Images
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Western blot - Anti-GNAO1 antibody (ab154001)Anti-GNAO1 antibody (ab154001) at 1/10000 dilution + Mouse brain whole cell
at 5 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Western blot - Anti-GNAO1 antibody (ab154001)All lanes : Anti-GNAO1 antibody (ab154001) at 1/1000 dilution
Lane 1 : HeLa whole cell
lysate
Lane 2 : NT2D1 whole cell
lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GNAO1 antibody (ab154001)Immunofluorescent analysis of methanol-fixed HeLa cells labeling GNAO1 with ab154001 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody (ab154001)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling GNAO1 with ab154001 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154001 has not yet been referenced specifically in any publications.