Overview

  • Product name

  • Description

    Rabbit polyclonal to GNAO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human GNAO1 aa 104-338.

  • Positive control

    • A549, HeLa, Jurkat, NT2D1 and mouse brain lysates; HeLa cells; Human lung adenocarcinoma tissue.
  • General notes

     This product was previously labelled as G0 Protein alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-GNAO1 antibody (ab154001) at 1/10000 dilution + Mouse brain whole cell
    at 5 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • All lanes : Anti-GNAO1 antibody (ab154001) at 1/1000 dilution

    Lane 1 : HeLa whole cell
    lysate
    Lane 2 : NT2D1 whole cell
    lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling GNAO1 with ab154001 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling GNAO1 with ab154001 at 1/500 dilution.

References

ab154001 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154001.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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