Anti-GNAQ antibody (ab128060)
Key features and details
- Goat polyclonal to GNAQ
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GNAQ antibody
See all GNAQ primary antibodies -
Description
Goat polyclonal to GNAQ -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Hamster, Cow, Dog, Pig, Monkey, a wide range of other species -
Immunogen
Synthetic peptide:
NDLDRVADPAYLPT
with a Cysteine residue linker, corresponding to internal sequence amino acids 162-175 of Human GNAQ. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 42 kDa).
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 42 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. -
Tissue specificity
Predominantly expressed in ovary, prostate, testis and colon. -
Sequence similarities
Belongs to the G-alpha family. G(q) subfamily. - Information by UniProt
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Database links
- Entrez Gene: 536654 Cow
- Entrez Gene: 403928 Dog
- Entrez Gene: 2776 Human
- Entrez Gene: 654405 Pig
- Entrez Gene: 81666 Rat
- Omim: 600998 Human
- SwissProt: Q28294 Dog
- SwissProt: P50148 Human
see all -
Alternative names
- CMC1 antibody
- G alpha Q antibody
- G protein alpha Q antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab128060 has been referenced in 6 publications.
- Kawakami K et al. Heterotrimeric Gq proteins act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias. Nat Commun 13:487 (2022). PubMed: 35078997
- Kusumoto J et al. OPN4 belongs to the photosensitive system of the human skin. Genes Cells 25:215-225 (2020). PubMed: 31989708
- Inoue A et al. Illuminating G-Protein-Coupling Selectivity of GPCRs. Cell 177:1933-1947.e25 (2019). PubMed: 31160049
- Chiang NY et al. GPR56/ADGRG1 Activation Promotes Melanoma Cell Migration via NTF Dissociation and CTF-Mediated Ga12/13/RhoA Signaling. J Invest Dermatol 137:727-736 (2017). PubMed: 27818281
- Devost D et al. Conformational Profiling of the AT1 Angiotensin II Receptor Reflects Biased Agonism, G Protein Coupling, and Cellular Context. J Biol Chem 292:5443-5456 (2017). PubMed: 28213525
- Hughes S et al. Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. Cell Mol Life Sci 72:165-79 (2015). IHC . PubMed: 24958088