Overview

  • Product name

  • Description

    Goat polyclonal to GNAQ
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster, Cow, Dog, Pig, Monkey, a wide range of other species
  • Immunogen

    Synthetic peptide:

    NDLDRVADPAYLPT

    with a Cysteine residue linker, corresponding to internal sequence amino acids 162-175 of Human GNAQ.

  • Positive control

    • Human, Mouse and Rat Testis lysates; Human Kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab128060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/64000.

Target

Images

  • Anti-GNAQ antibody (ab128060) at 0.1 µg/ml + Rat Testis lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • ab128060, at 5 µg/ml, staining GNAQ in paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Chiang NY  et al. GPR56/ADGRG1 Activation Promotes Melanoma Cell Migration via NTF Dissociation and CTF-Mediated Ga12/13/RhoA Signaling. J Invest Dermatol 137:727-736 (2017). Read more (PubMed: 27818281) »
  • Devost D  et al. Conformational Profiling of the AT1 Angiotensin II Receptor Reflects Biased Agonism, G Protein Coupling, and Cellular Context. J Biol Chem 292:5443-5456 (2017). Read more (PubMed: 28213525) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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