Key features and details
- Rabbit polyclonal to GNAQ
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GNAQ antibody
See all GNAQ primary antibodies
DescriptionRabbit polyclonal to GNAQ
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
Recombinant fragment within Human GNAQ (internal sequence). The exact sequence is proprietary.
Database link: P50148
- IHC-P: Human placenta tissue; Rat intestine tissue; Mouse prostate and ovary tissues. WB: SK-N-SH whole cell extract; GNAQ-transfected HEK-293T whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Tissue specificityPredominantly expressed in ovary, prostate, testis and colon.
Sequence similaritiesBelongs to the G-alpha family. G(q) subfamily.
- Information by UniProt
- CMC1 antibody
- G alpha Q antibody
- G protein alpha Q antibody
Paraffin-embedded human placenta tissue stained for GNAQ using ab227123 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded rat intestine tissue stained for GNAQ using ab227123 at 1/500 dilution in immunohistochemical analysis.
Anti-GNAQ antibody (ab227123) at 1/500 dilution + SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 42 kDa
10% SDS-PAGE gel.
All lanes : Anti-GNAQ antibody (ab227123) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : 3xDDDDK-human GNAQ-transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10 % SDS-PAGE gel.
Paraffin-embedded mouse prostate tissue stained for GNAQ using ab227123 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse ovary tissue stained for GNAQ using ab227123 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227123 has not yet been referenced specifically in any publications.