Overview

  • Product name

    Anti-GNAQ antibody [EPR17149]
    See all GNAQ primary antibodies
  • Description

    Rabbit monoclonal [EPR17149] to GNAQ
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 77% homology with GNA11. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GNAQ aa 50-150. The exact sequence is proprietary.
    Database link: P50148

  • Positive control

    • HEK293, HeLa, HepG2, C6, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, mouse kidney, mouse spleen, rat brain and rat kidney lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
  • Tissue specificity

    Predominantly expressed in ovary, prostate, testis and colon.
  • Sequence similarities

    Belongs to the G-alpha family. G(q) subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMC1 antibody
    • G alpha Q antibody
    • G protein alpha Q antibody
    • G protein subunit alpha q antibody
    • G-ALPHA-q antibody
    • GAQ antibody
    • GNAQ antibody
    • GNAQ_HUMAN antibody
    • guanine nucleotide binding protein (G protein), q polypeptide antibody
    • Guanine nucleotide binding protein alpha q antibody
    • guanine nucleotide binding protein G protein q polypeptide antibody
    • Guanine nucleotide binding protein G q subunit alpha antibody
    • Guanine nucleotide-binding protein alpha-q antibody
    • Guanine nucleotide-binding protein G(q) subunit alpha antibody
    • SWS antibody
    see all

Images

  • All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution

    Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-GNAQ antibody [EPR17149] (ab199533) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

See 1 Publication for this product

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