Anti-GNAS antibody [7G6G5] (ab216129)
Key features and details
- Mouse monoclonal [7G6G5] to GNAS
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-GNAS antibody [7G6G5]
See all GNAS primary antibodies -
Description
Mouse monoclonal [7G6G5] to GNAS -
Host species
Mouse -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human G protein alpha aa 42-188. Expressed in E. Coli.
Database link: P63092 -
Positive control
- WB: Human G protein alpha (aa: 42-188) recombinant protein; Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate. ICC/IF: HeLa cells. FACS: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7G6G5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
ICC/IF |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
|
|
ICC/IF |
1/200 - 1/1000.
|
|
Flow Cyt |
1/200 - 1/400.
|
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
Target
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Relevance
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 2778 Human
- Omim: 139320 Human
- SwissProt: P63092 Human
- SwissProt: P84996 Human
- SwissProt: Q5JWF2 Human
- Unigene: 125898 Human
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Alternative names
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
see all
Images
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Flow cytometric analysis of MCF7 cells labeling G protein alpha with ab216129 at 1/200 dilution (green). Negative control (red).
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Immunofluorescent analaysis of HeLa cells labeling G protein alpha with ab216129 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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All lanes : Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution
Lane 1 : non-transfected HEK293 cell lysate
Lane 2 : Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 45 kDa -
Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution + Human G protein alpha (aa: 42-188) recombinant protein
Predicted band size: 45 kDaExpected MW is 42.8 kDa
Protocols
Datasheets and documents
References (0)
ab216129 has not yet been referenced specifically in any publications.