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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins
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Anti-GNAS antibody [7G6G5] (ab216129)

  • Datasheet
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Flow Cytometry - Anti-GNAS antibody [7G6G5] (ab216129)
  • Immunocytochemistry/ Immunofluorescence - Anti-GNAS antibody [7G6G5] (ab216129)
  • Western blot - Anti-GNAS antibody [7G6G5] (ab216129)
  • Western blot - Anti-GNAS antibody [7G6G5] (ab216129)

Key features and details

  • Mouse monoclonal [7G6G5] to GNAS
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human GNAS protein (ab158540)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-GNAS antibody [7G6G5]
    See all GNAS primary antibodies
  • Description

    Mouse monoclonal [7G6G5] to GNAS
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human G protein alpha aa 42-188. Expressed in E. Coli.
    Database link: P63092

  • Positive control

    • WB: Human G protein alpha (aa: 42-188) recombinant protein; Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate. ICC/IF: HeLa cells. FACS: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    7G6G5
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • G Proteins
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human GNAS protein (ab158540)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 45 kDa.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

Target

  • Relevance

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
  • Cellular localization

    Cell Membrane
  • Database links

    • Entrez Gene: 2778 Human
    • Omim: 139320 Human
    • SwissProt: P63092 Human
    • SwissProt: P84996 Human
    • SwissProt: Q5JWF2 Human
    • Unigene: 125898 Human
    • Alternative names

      • Adenylate cyclase stimulating G alpha protein antibody
      • AHO antibody
      • Alternative gene product encoded by XL exon antibody
      • C20orf45 antibody
      • dJ309F20.1.1 antibody
      • dJ806M20.3.3 antibody
      • Extra large alphas protein antibody
      • GNAS antibody
      • GNAS complex locus antibody
      • GNAS1 antibody
      • GPSA antibody
      • Gs alpha subunit antibody
      • GSA antibody
      • GSP antibody
      • Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1 antibody
      • Guanine nucleotide binding protein alpha stimulating activity polypeptide 1 antibody
      • Guanine nucleotide binding protein G(s) subunit alpha isoforms short antibody
      • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas antibody
      • Guanine nucleotide regulatory protein antibody
      • MGC33735 antibody
      • NESP antibody
      • NESP55 antibody
      • Neuroendocrine secretory protein antibody
      • PHP1A antibody
      • PHP1B antibody
      • PHP1C antibody
      • POH antibody
      • Protein ALEX antibody
      • Protein GNAS antibody
      • Protein NESP55 antibody
      • SCG6 antibody
      • Secretogranin VI antibody
      • XLalphas antibody
      • XLas antibody
      see all

    Images

    • Flow Cytometry - Anti-GNAS antibody [7G6G5] (ab216129)
      Flow Cytometry - Anti-GNAS antibody [7G6G5] (ab216129)

      Flow cytometric analysis of MCF7 cells labeling G protein alpha with ab216129 at 1/200 dilution (green). Negative control (red).

    • Immunocytochemistry/ Immunofluorescence - Anti-GNAS antibody [7G6G5] (ab216129)
      Immunocytochemistry/ Immunofluorescence - Anti-GNAS antibody [7G6G5] (ab216129)

      Immunofluorescent analaysis of HeLa cells labeling G protein alpha with ab216129 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

    • Western blot - Anti-GNAS antibody [7G6G5] (ab216129)
      Western blot - Anti-GNAS antibody [7G6G5] (ab216129)
      All lanes : Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution

      Lane 1 : non-transfected HEK293 cell lysate
      Lane 2 : Human G protein alpha (aa: 42-188)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 45 kDa

    • Western blot - Anti-GNAS antibody [7G6G5] (ab216129)
      Western blot - Anti-GNAS antibody [7G6G5] (ab216129)
      Anti-GNAS antibody [7G6G5] (ab216129) at 1/500 dilution + Human G protein alpha (aa: 42-188) recombinant protein

      Predicted band size: 45 kDa



      Expected MW is 42.8 kDa

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab216129? Please let us know so that we can cite the reference in this datasheet.

    ab216129 has not yet been referenced specifically in any publications.

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