Overview

  • Product name

  • Description

    Rabbit polyclonal to GNAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Pig
  • Immunogen

    A region within synthetic peptide: YRATHRLLLL GAGESGKSTI VKQMRILHVN GFNGDSEKAT KVQDIKNNLK, corresponding to N terminal amino acids 37-86 of Human G protein alpha

  • Positive control

    • WB: HepG2 membrane lysate IHC-P: Human kidney tissue and human lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • Adenylate cyclase stimulating G alpha protein antibody
    • AHO antibody
    • Alternative gene product encoded by XL exon antibody
    • C20orf45 antibody
    • dJ309F20.1.1 antibody
    • dJ806M20.3.3 antibody
    • Extra large alphas protein antibody
    • GNAS antibody
    • GNAS complex locus antibody
    • GNAS1 antibody
    • GPSA antibody
    • Gs alpha subunit antibody
    • GSA antibody
    • GSP antibody
    • Guanine nucleotide binding protein (G protein) alpha stimulating activity polypeptide 1 antibody
    • Guanine nucleotide binding protein alpha stimulating activity polypeptide 1 antibody
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms short antibody
    • Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas antibody
    • Guanine nucleotide regulatory protein antibody
    • MGC33735 antibody
    • NESP antibody
    • NESP55 antibody
    • Neuroendocrine secretory protein antibody
    • PHP1A antibody
    • PHP1B antibody
    • PHP1C antibody
    • POH antibody
    • Protein ALEX antibody
    • Protein GNAS antibody
    • Protein NESP55 antibody
    • SCG6 antibody
    • Secretogranin VI antibody
    • XLalphas antibody
    • XLas antibody
    see all

Images

  • Anti-GNAS antibody (ab58916) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 46 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling G-protein alpha with ab58916 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling G-protein alpha with ab58916 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

References

This product has been referenced in:

  • Kim JE  et al. Effects of five candidate laxatives derived from Liriope platyphylla on the 5-HT receptor signaling pathway in three cell types present in the transverse colon. Mol Med Rep 15:431-441 (2017). Read more (PubMed: 27922695) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - other (whole brain lysate)
Loading amount
20 µg
Specification
whole brain lysate
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 12 2010

Answer

Thank you for your inquiry and patience. ab58651 and ab58916 are different peptides against GNAS complex. Human Gnas1 and Gnas2 have 100% and 98% homology, respective to antibodies. The homology is very low against other subunits but the cross-reactivity has not been tested. ab34027-(ab34030 BSA and azide free version). This antibdy has been shown as stated on the datasheet not to react with some of the subunits. I apologize if it seemed that the antibodies are anti- pan-protein G alpha antibodies against all subunits. But I assume as long as the specific subunits are present the antibodies recognize the G-alpha complex. I hope this helps. Please let me know if you have further questions.

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