• Product name
  • Description
    Rabbit polyclonal to GNAT1
  • Host species
  • Specificity
    Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Cow, Pig
    Predicted to work with: Rat, Dog, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human Transducin alpha aa 18-31.


    (Peptide available as ab5002)


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).Can be blocked with GNAT1 peptide (ab5002).


  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • Tissue specificity
    Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.
  • Involvement in disease
    Night blindness, congenital stationary, autosomal dominant 3
    Night blindness, congenital stationary, 1C
  • Sequence similarities
    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Developmental stage
    Expressed at approximately postnatal day 7.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSNBAD3 antibody
    • GBT1 antibody
    • GNAT1 antibody
    • GNAT1_HUMAN antibody
    • GNATR antibody
    • guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 1 antibody
    • guanine nucleotide binding protein G(T) alpha 1 subunit antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-1 antibody
    • Rod specific transducin antibody
    • rod-type transducin alpha subunit antibody
    • Transducin alpha-1 chain antibody
    • transducin, rod-specific antibody
    see all


  • Western blot detection of Trabsducin alpha on sheep retinal extracts using ab3504.


This product has been referenced in:
  • Taniuchi K  et al. Overexpression of GalNAc-transferase GalNAc-T3 promotes pancreatic cancer cell growth. Oncogene 30:4843-54 (2011). Read more (PubMed: 21625220) »
  • Adamus G  et al. Molecular biomarkers for autoimmune retinopathies: significance of anti-transducin-alpha autoantibodies. Exp Mol Pathol 87:195-203 (2009). Read more (PubMed: 19744478) »
See all 2 Publications for this product

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