Overview

  • Product name

  • Description

    Rabbit polyclonal to GNAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthesized peptide derived from the internal of human Transducin alpha

  • Positive control

    • Extracts from COLO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/40000.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • Tissue specificity

    Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.
  • Involvement in disease

    Night blindness, congenital stationary, autosomal dominant 3
    Night blindness, congenital stationary, 1C
  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Developmental stage

    Expressed at approximately postnatal day 7.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSNBAD3 antibody
    • GBT1 antibody
    • GNAT1 antibody
    • GNAT1_HUMAN antibody
    • GNATR antibody
    • guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 1 antibody
    • guanine nucleotide binding protein G(T) alpha 1 subunit antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-1 antibody
    • Rod specific transducin antibody
    • rod-type transducin alpha subunit antibody
    • Transducin alpha-1 chain antibody
    • transducin, rod-specific antibody
    see all

Images

  • All lanes : Anti-GNAT1 antibody (ab74059) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa
    Additional bands at: 48 kDa, 70 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Szabó K  et al. Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats. Sci Rep 7:8891 (2017). Read more (PubMed: 28827737) »
  • Szabó K  et al. Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina. Cell Tissue Res 356:49-63 (2014). Read more (PubMed: 24496510) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I am sorry that this product has not been performing as stated on the datasheet and for the inconvenience this has caused. I would be very happy to issue a free of charge replacement for this product provide that the vial was still within our six month Abpromise guarantee period when you first contacted us. In order to process this free of charge replacement could you please send to me the original Abcam order confirmation number or alternatively, the PO number and date of purchase. I would aslo like to let you know that right now Abcam has a promotion for a free Rabbit Monoclonal antibody with purchase of a primary antibody. I would like to offer this promotion to you with this free of charge replacement. If there is any RabMAb which you would like, please let me know and I will include this free. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

I am very sorry to hear that you have been experiencing difficulties when using this product. In order that I may more fully understand the problem, and so that we may have the most complete records for the product, could you please provide me with the details of your experiments including sample prep, prtocol and tropubleshooting?

This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise



I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin, Retina)
Specification
Skin, Retina
Fixative
Methanol
Permeabilization
Yes - 0.4% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

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Verified customer

Submitted Nov 09 2011

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