Anti-GNAT1 antibody (ab74059)
Key features and details
- Rabbit polyclonal to GNAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GNAT1 antibody
See all GNAT1 primary antibodies -
Description
Rabbit polyclonal to GNAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized peptide derived from the internal of human Transducin alpha
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 40 kDa. |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. -
Tissue specificity
Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium. -
Involvement in disease
Night blindness, congenital stationary, autosomal dominant 3
Night blindness, congenital stationary, 1C -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Developmental stage
Expressed at approximately postnatal day 7. - Information by UniProt
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Database links
- Entrez Gene: 2779 Human
- Omim: 139330 Human
- SwissProt: P11488 Human
- Unigene: 517978 Human
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Alternative names
- CSNBAD3 antibody
- GBT1 antibody
- GNAT1 antibody
see all
Images
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All lanes : Anti-GNAT1 antibody (ab74059) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 48 kDa, 70 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab74059 has been referenced in 10 publications.
- Striebel JF et al. Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses. Acta Neuropathol Commun 9:17 (2021). PubMed: 33509294
- Nishiguchi KM et al. Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice. Nat Commun 11:482 (2020). PubMed: 31980606
- Barnea-Cramer AO et al. Repair of Retinal Degeneration following Ex Vivo Minicircle DNA Gene Therapy and Transplantation of Corrected Photoreceptor Progenitors. Mol Ther 28:830-844 (2020). PubMed: 32027843
- Cammalleri M et al. The urokinase-type plasminogen activator system as drug target in retinitis pigmentosa: New pre-clinical evidence in the rd10 mouse model. J Cell Mol Med 23:5176-5192 (2019). PubMed: 31251468
- Zhang X et al. Disease mechanisms and neuroprotection by tauroursodeoxycholic acid in Rpgr knockout mice. J Cell Physiol 234:18801-18812 (2019). PubMed: 30924157
- Nishiguchi KM et al. Retained Plasticity and Substantial Recovery of Rod-Mediated Visual Acuity at the Visual Cortex in Blind Adult Mice with Retinal Dystrophy. Mol Ther 26:2397-2406 (2018). PubMed: 30064895
- Szabó K et al. Histological Evaluation of Diabetic Neurodegeneration in the Retina of Zucker Diabetic Fatty (ZDF) Rats. Sci Rep 7:8891 (2017). PubMed: 28827737
- Szabó K et al. Immunocytochemical analysis of misplaced rhodopsin-positive cells in the developing rodent retina. Cell Tissue Res 356:49-63 (2014). PubMed: 24496510
- Chucair-Elliott AJ et al. Leukemia Inhibitory Factor (LIF) coordinates the downregulation of the visual cycle in the retina and retinal pigmented epithelium (RPE). J Biol Chem : (2012). WB ; Mouse . PubMed: 22645143
- Goto M et al. Phosphodiesterase inhibitors block the acceleration of skin permeability barrier repair by red light. Exp Dermatol 20:568-71 (2011). IHC-Fr ; Mouse . PubMed: 21410772