Overview

  • Product name

  • Description

    Rabbit polyclonal to GNAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse GNAT2 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab110444)

  • Positive control

    • This antibody gave a positive signal in both Mouse and Rat retina tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab110214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • Tissue specificity

    Retinal rod outer segment.
  • Involvement in disease

    Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
  • Sequence similarities

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACHM4 antibody
    • Cone type transducin alpha subunit antibody
    • GNAT 2 antibody
    • GNAT C antibody
    • Gnat2 antibody
    • GNAT2_HUMAN antibody
    • GNATC antibody
    • Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2 antibody
    • Guanine nucleotide binding protein G t subunit alpha 2 antibody
    • Guanine nucleotide-binding protein G(t) subunit alpha-2 antibody
    • Transducin alpha 2 antibody
    • Transducin alpha-2 chain antibody
    • Transducin alpha2 antibody
    • Transducin cone specific alpha polypeptide antibody
    see all

Images

  • All lanes : Anti-GNAT2 antibody (ab110214) at 1 µg/ml

    Lane 1 : Mouse Retina Tissue Lysate
    Lane 2 : Rat Retina Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes

References

This product has been referenced in:

  • Nagaraj R  et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell 168:210-223.e11 (2017). Read more (PubMed: 28086092) »
See 1 Publication for this product

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