Key features and details
- Rabbit polyclonal to GNAT2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-GNAT2 antibody
See all GNAT2 primary antibodies
DescriptionRabbit polyclonal to GNAT2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Dog, Human
Synthetic peptide corresponding to Mouse GNAT2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both Mouse and Rat retina tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab110214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
Tissue specificityRetinal rod outer segment.
Involvement in diseaseDefects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
Sequence similaritiesBelongs to the G-alpha family. G(i/o/t/z) subfamily.
- Information by UniProt
- ACHM4 antibody
- Cone type transducin alpha subunit antibody
- GNAT 2 antibody
All lanes : Anti-GNAT2 antibody (ab110214) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
ab110214 has been referenced in 1 publication.
- Nagaraj R et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell 168:210-223.e11 (2017). PubMed: 28086092