Overview

  • Product name
    Anti-GNAT2 antibody
    See all GNAT2 primary antibodies
  • Description
    Rabbit polyclonal to GNAT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GNAT2.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in both Rat and Mouse Retina tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
  • Tissue specificity
    Retinal rod outer segment.
  • Involvement in disease
    Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination.
  • Sequence similarities
    Belongs to the G-alpha family. G(i/o/t/z) subfamily.
  • Information by UniProt
  • Database links
    • Alternative names
      • ACHM4 antibody
      • Cone type transducin alpha subunit antibody
      • GNAT 2 antibody
      • GNAT C antibody
      • Gnat2 antibody
      • GNAT2_HUMAN antibody
      • GNATC antibody
      • Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2 antibody
      • Guanine nucleotide binding protein G t subunit alpha 2 antibody
      • Guanine nucleotide-binding protein G(t) subunit alpha-2 antibody
      • Transducin alpha 2 antibody
      • Transducin alpha-2 chain antibody
      • Transducin alpha2 antibody
      • Transducin cone specific alpha polypeptide antibody
      see all

    Images

    • Western blot - Anti-GNAT2 antibody (ab97501)
      Western blot - Anti-GNAT2 antibody (ab97501)
      All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml

      Lane 1 : Mouse Retina Tissue Lysate
      Lane 2 : Rat Retina Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 54 kDa (why is the actual band size different from the predicted?)


      Exposure time: 1 minute

    References

    This product has been referenced in:
    • Datta P  et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . Read more (PubMed: 26216965) »
    • Sato K  et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . Read more (PubMed: 22736942) »

    See all 2 Publications for this product

    Publishing research using ab97501? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-GNAT2 antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Retinal)
    Loading amount
    10 µg
    Specification
    Retinal
    Gel Running Conditions
    Reduced Denaturing (10% Tris Glycine Gel)
    Blocking step
    1:1 PBS: Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
    Read More
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    Username

    Dr. Chris Tracy

    Verified customer

    Submitted Aug 24 2012

    What is the immunogen seq for this antibody?

    Thank you for calling Abcam earlier today,

    Please find the immunogen sequence for ab97501 below:
    ********

    If there is anything else I can help you with.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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