Anti-GNAT2 antibody (ab97501)
Key features and details
- Rabbit polyclonal to GNAT2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GNAT2 antibody
See all GNAT2 primary antibodies -
Description
Rabbit polyclonal to GNAT2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in both Rat and Mouse Retina tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. -
Tissue specificity
Retinal rod outer segment. -
Involvement in disease
Defects in GNAT2 are the cause of achromatopsia type 4 (ACHM4) [MIM:139340]. Achromatopsia is an autosomal recessively inherited visual disorder that is present from birth and that features the absence of color discrimination. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. - Information by UniProt
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Database links
- Entrez Gene: 14686 Mouse
- Entrez Gene: 365901 Rat
- SwissProt: P50149 Mouse
- Unigene: 439652 Mouse
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Alternative names
- ACHM4 antibody
- Cone type transducin alpha subunit antibody
- GNAT 2 antibody
see all
Images
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Western blot - Anti-GNAT2 antibody (ab97501)All lanes : Anti-GNAT2 antibody (ab97501) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab97501 has been referenced in 4 publications.
- Datta P et al. Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance. PLoS One 16:e0246358 (2021). PubMed: 33961633
- Maggi EC et al. Retinoblastoma-binding protein 2 (RBP2) is frequently expressed in neuroendocrine tumors and promotes the neoplastic phenotype. Oncogenesis 5:e257 (2016). PubMed: 27548814
- Datta P et al. Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Proc Natl Acad Sci U S A 112:E4400-9 (2015). WB ; Mouse . PubMed: 26216965
- Sato K et al. M-opsin protein degradation is inhibited by MG-132 in Rpe65(-/-) retinal explant culture. Mol Vis 18:1516-25 (2012). WB ; Mouse . PubMed: 22736942