Anti-GNAT3 antibody (ab113664)
Key features and details
- Goat polyclonal to GNAT3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GNAT3 antibody -
Description
Goat polyclonal to GNAT3 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig -
Immunogen
Synthetic peptide:
C-KNQFLDLNLKKEDKE
(with a Cysteine residue linker), corresponding to internal sequence amino acids 304-318 of Human GNAT3 (NP_001095856.1) -
Positive control
- Human Small Intestine
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. -
Tissue specificity
Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa. -
Sequence similarities
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
Post-translational
modificationsPotential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane. -
Cellular localization
Cytoplasm. Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 346562 Human
- Entrez Gene: 242851 Mouse
- Entrez Gene: 286924 Rat
- Omim: 139395 Human
- SwissProt: A8MTJ3 Human
- SwissProt: Q3V3I2 Mouse
- SwissProt: P29348 Rat
- Unigene: 335049 Human
see all -
Alternative names
- GDCA antibody
- Ggust antibody
- Gnat 3 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (4)
ab113664 has been referenced in 4 publications.
- Ito M et al. Morphology and chemical characteristics of taste buds associated with P2X3-immunoreactive afferent nerve endings in the rat incisive papilla. J Anat 240:688-699 (2022). PubMed: 34719779
- Hoffman MT et al. The Gustatory Sensory G-Protein GNAT3 Suppresses Pancreatic Cancer Progression in Mice. Cell Mol Gastroenterol Hepatol 11:349-369 (2021). PubMed: 32882403
- Yamamoto Y et al. Morphology of GNAT3-immunoreactive chemosensory cells in the nasal cavity and pharynx of the rat. J Anat 239:290-306 (2021). PubMed: 33677835
- Masuda H et al. Morphology of GNAT3-immunoreactive chemosensory cells in the rat larynx. J Anat 234:149-164 (2019). PubMed: 30467855