Overview

  • Product name

  • Description

    Rabbit polyclonal to GNB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 67-271 of Human GNB1.

  • Positive control

    • A431 whole cell lysate (ab7909) can be used as a positive control in WB. Jurkat whole cells lysate; Mouse brain whole cell lysate; methanol-fixed HeLa cells; Lung papillory Adeno Carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab137635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 37 kDa.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
  • Sequence similarities

    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta subunit signal transducing proteins GS/GI antibody
    • G protein beta 1 subunit antibody
    • GBB1 antibody
    • GBB1_HUMAN antibody
    • gnb1 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 1 antibody
    • Guanine nucleotide binding protein beta 1 subunit antibody
    • Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 antibody
    • Transducin beta chain 1 antibody
    see all

Images

  • Anti-GNB1 antibody (ab137635) at 1/5000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS Page
  • Immunohistochemical analysis of paraffin-embedded Lung papillory Adeno Carcinoma, labelling GNB1 using ab137635 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labelling GNB1 using ab137635 at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.
  • Anti-GNB1 antibody (ab137635) at 1/10000 dilution + Mouse brain whole cells lysate at 50 µg

    Predicted band size: 37 kDa



    10% SDS Page

References

This product has been referenced in:

  • Murakami T  et al. Cutting Edge: G Protein Subunit ß 1 Negatively Regulates NLRP3 Inflammasome Activation. J Immunol 202:1942-1947 (2019). Read more (PubMed: 30777924) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137635.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up