Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3262Y] to GNB2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-GNB2 antibody [EP3262Y] - BSA and Azide free
See all GNB2 primary antibodies
DescriptionRabbit monoclonal [EP3262Y] to GNB2 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GNB2 aa 250-350 (C terminal). The exact sequence is proprietary.
- WB: HeLa, Jurkat and SH-SY5Y cell lysates.
Ab247673 is the carrier-free version of ab108504. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247673 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- G protein beta 2 subunit antibody
- G protein subunit beta 2 antibody
- G protein subunit beta-2 antibody
All lanes : Anti-GNB2 antibody [EP3262Y] (ab108504) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GNB2 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab108504).
ab108504 Anti-GNB2 antibody [EP3262Y] was shown to specifically react with GNB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266528 (knockout cell lysate ab257453) was used. Wild-type and GNB2 knockout samples were subjected to SDS-PAGE. ab108504 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247673 has not yet been referenced specifically in any publications.