Product nameAnti-GNB2 antibody [EPR3261Y]
See all GNB2 primary antibodies
DescriptionRabbit monoclonal [EPR3261Y] to GNB2
Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, IPmore details
Unsuitable for: ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GNB2 aa 1-100 (N terminal). The exact sequence is proprietary.
- SHSY5Y, Jurkat and NIH3T3 cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab81272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 37 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- G protein beta 2 subunit antibody
- G protein subunit beta 2 antibody
- G protein subunit beta-2 antibody
All lanes : Anti-GNB2 antibody [EPR3261Y] (ab81272) at 1/20000 dilution
Lane 1 : SHSY5Y cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : NIH3T3 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?