Key features and details
- Rabbit polyclonal to GNB4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GNB4 antibody
DescriptionRabbit polyclonal to GNB4
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: Rat liver tissue lysate; Mouse liver and kidney tissue lysates. IHC-P: Human pancreatic and colon cancer tissues. ICC/IF: HeLa cells.
This product was previously labelled as G protein beta 4
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab223113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionGuanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Tissue specificityStrongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart.
Sequence similaritiesBelongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
- Information by UniProt
- G protein beta 4 subunit antibody
- GBB4_HUMAN antibody
- GNB 4 antibody
All lanes : Anti-GNB4 antibody (ab223113) at 1/500 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Paraffin-embedded human pancreatic cancer tissue stained for GNB4 using ab223113 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for GNB4 using ab223113 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GNB4 (green) using ab223113 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
ab223113 has not yet been referenced specifically in any publications.