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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins
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Anti-GNB4 antibody (ab223113)

  • Datasheet
  • SDS
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Abpromise

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Western blot - Anti-GNB4 antibody (ab223113)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB4 antibody (ab223113)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB4 antibody (ab223113)
  • Immunocytochemistry/ Immunofluorescence - Anti-GNB4 antibody (ab223113)

Key features and details

  • Rabbit polyclonal to GNB4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human GNB4 protein (ab40639)

View more associated products

Overview

  • Product name

    Anti-GNB4 antibody
  • Description

    Rabbit polyclonal to GNB4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human GNB4 aa 105-185.
    Sequence:

    YAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELPGHTGYLSCCRFLDD SQIVTSSGDTTCALWDIETAQQTTTFTGHSG


    Database link: Q9HAV0
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat liver tissue lysate; Mouse liver and kidney tissue lysates. IHC-P: Human pancreatic and colon cancer tissues. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as G protein beta 4

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • G Proteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
    • Mouse kidney normal tissue lysate - total protein (ab29305)
  • Recombinant Protein

    • Recombinant Human GNB4 protein (ab40639)
  • Related Products

    • Recombinant Human GNB4 protein (ab40639)

Applications

Our Abpromise guarantee covers the use of ab223113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
  • Tissue specificity

    Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart.
  • Sequence similarities

    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Target information above from: UniProt accession Q9HAV0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 59345 Human
    • Entrez Gene: 14696 Mouse
    • Entrez Gene: 294962 Rat
    • Omim: 610863 Human
    • SwissProt: Q9HAV0 Human
    • SwissProt: P29387 Mouse
    • SwissProt: Q3THF3 Mouse
    • SwissProt: Q3TY18 Mouse
    • SwissProt: Q3U2F9 Mouse
    • SwissProt: O35353 Rat
    • SwissProt: Q4V8I8 Rat
    • Unigene: 173030 Human
    • Unigene: 139192 Mouse
    • Unigene: 10844 Rat
    see all
  • Alternative names

    • G protein beta 4 subunit antibody
    • GBB4_HUMAN antibody
    • GNB 4 antibody
    • Gnb4 antibody
    • Guanine nucleotide binding protein (G protein) beta polypeptide 4 antibody
    • Guanine nucleotide binding protein beta 4 subunit antibody
    • Guanine nucleotide binding protein beta polypeptide 4 antibody
    • Guanine nucleotide binding protein beta subunit 4 antibody
    • Guanine nucleotide binding protein subunit beta 4 antibody
    • Guanine nucleotide-binding protein subunit beta-4 antibody
    • Transducin beta chain 4 antibody
    see all

Images

  • Western blot - Anti-GNB4 antibody (ab223113)
    Western blot - Anti-GNB4 antibody (ab223113)
    All lanes : Anti-GNB4 antibody (ab223113) at 1/500 dilution

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse kidney tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB4 antibody (ab223113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB4 antibody (ab223113)

    Paraffin-embedded human pancreatic cancer tissue stained for GNB4 using ab223113 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB4 antibody (ab223113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB4 antibody (ab223113)

    Paraffin-embedded human colon cancer tissue stained for GNB4 using ab223113 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-GNB4 antibody (ab223113)
    Immunocytochemistry/ Immunofluorescence - Anti-GNB4 antibody (ab223113)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GNB4 (green) using ab223113 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab223113? Please let us know so that we can cite the reference in this datasheet.

    ab223113 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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