RecombinantRabMAb

Recombinant Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)

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Western blot - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
  • Flow Cytometry - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
  • Western blot - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
  • Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15058] to GNE - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GNE antibody [EPR15058] - BSA and Azide free
    See all GNE primary antibodies

  • Description

    Rabbit monoclonal [EPR15058] to GNE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GNE aa 50-150. The exact sequence is proprietary.
    Database link: Q9Y223

  • General notes

    ab250798 is the carrier-free version of ab184963 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab250798 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as GLCNE

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab250798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).

Target

  • Function

    Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.

  • Tissue specificity

    Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon.

  • Pathway

    Amino-sugar metabolism; N-acetylneuraminate biosynthesis.

  • Involvement in disease

    Defects in GNE are a cause of sialuria (SIALURIA) [MIM:269921]; also known as sialuria French type. In sialuria, free sialic acid accumulates in the cytoplasm and gram quantities of neuraminic acid are secreted in the urine. The metabolic defect involves lack of feedback inhibition of UDP-GlcNAc 2-epimerase by CMP-Neu5Ac, resulting in constitutive overproduction of free Neu5Ac. Clinical features include variable degrees of developmental delay, coarse facial features and hepatomegaly. Sialuria inheritance is autosomal dominant.
    Defects in GNE are the cause of inclusion body myopathy type 2 (IBM2) [MIM:600737]. Hereditary inclusion body myopathies are a group of neuromuscular disorders characterized by adult onset, slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM2 is an autosomal recessive disorder affecting mainly leg muscles, but with an unusual distribution that spares the quadriceps as also observed in Nonaka myopathy.
    Defects in GNE are the cause of Nonaka myopathy (NM) [MIM:605820]; also known as distal myopathy with rimmed vacuoles (DMRV). NM is an autosomal recessive muscular disorder, allelic to inclusion body myopathy 2. It is characterized by weakness of the anterior compartment of the lower limbs with onset in early adulthood, and sparing of the quadriceps muscles. As the inclusion body myopathy, NM is histologically characterized by the presence of numerous rimmed vacuoles without inflammatory changes in muscle specimens.

  • Sequence similarities

    In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.
    In the C-terminal section; belongs to the ROK (NagC/XylR) family.

  • Post-translational
    modifications

    Phosphorylated by PKC.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2310066H07Rik antibody
    • Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase antibody
    • DMRV antibody
    • GLCNE_HUMAN antibody
    • Glucosamine (UDP N acetyl) 2 epimerase/N acetylmannosamine kinase antibody
    • GNE antibody
    • IBM2 antibody
    • ManAc kinase antibody
    • N acylmannosamine kinase antibody
    • N-acetylmannosamine kinase antibody
    • NM antibody
    • RP23-209M8.6 antibody
    • Uae1 antibody
    • UDP GlcNAc 2 epimerase antibody
    • UDP GlcNAc 2 epimerase/ManAc kinase antibody
    • UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase antibody
    • UDP-GlcNAc-2-epimerase antibody
    • UDP-GlcNAc-2-epimerase/ManAc kinase antibody
    • Uridine diphosphate N acetylglucosamine 2 epimerase antibody
    • Uridine diphosphate-N-acetylglucosamine-2-epimerase antibody
    see all

Images

  • Western blot - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    Western blot - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    All lanes : Anti-GNE antibody [EPR15058] (ab184963) at 1/10000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa



    This data was developed using ab184963, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    Flow Cytometry - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    This data was developed using ab184963, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling GNE with ab184963 at 1/100 dilution (red) or a Rabbit monoclonal IgG (negative) (green) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Western blot - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    Western blot - Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    Anti-GNE antibody [EPR15058] (ab184963) at 1/20000 dilution + SW480 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 79 kDa



    This data was developed using ab184963, the same antibody clone in a different buffer formulation.

     

  • Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)
    Anti-GNE antibody [EPR15058] - BSA and Azide free (ab250798)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250798? Please let us know so that we can cite the reference in this datasheet.

ab250798 has not yet been referenced specifically in any publications.

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