Overview

  • Product name

  • Description

    Rabbit polyclonal to GNL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide within Human GNL1 aa 50-100. The exact sequence is proprietary. NP_005266.2
    Database link: P36915

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245634 was affinity purified using an epitope specific to GNL1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.

Target

  • Function

    Possible regulatory or functional link with the histocompatibility cluster.
  • Sequence similarities

    Belongs to the MMR1/HSR1 GTP-binding protein family.
    Contains 1 G (guanine nucleotide-binding) domain.
  • Domain

    In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gnl1 antibody
    • GNL1_HUMAN antibody
    • GTP binding protein HSR1 antibody
    • GTP-binding protein HSR1 antibody
    • Guanine nucleotide binding protein like 1 antibody
    • Guanine nucleotide-binding protein-like 1 antibody
    • HSR1 antibody
    • HSR1 GTP binding protein antibody
    see all

Images

  • All lanes : Anti-GNL1 antibody (ab245634) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa


    Exposure time: 3 minutes
  • GNL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245634 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245634 at 0.4 µg/ml.

    Lane 1: ab245634 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab245634 has not yet been referenced specifically in any publications.

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