Key features and details
- Rabbit polyclonal to GNL1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GNL1 antibody
See all GNL1 primary antibodies
DescriptionRabbit polyclonal to GNL1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Orangutan
Synthetic peptide within Human GNL1 aa 50-100. The exact sequence is proprietary. NP_005266.2
Database link: P36915
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245634 was affinity purified using an epitope specific to GNL1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
FunctionPossible regulatory or functional link with the histocompatibility cluster.
Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain.
DomainIn contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Gnl1 antibody
- GNL1_HUMAN antibody
- GTP binding protein HSR1 antibody
All lanes : Anti-GNL1 antibody (ab245634) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes
GNL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245634 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245634 at 0.4 µg/ml.
Lane 1: ab245634 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245634 has not yet been referenced specifically in any publications.