Product nameAnti-GNL1 antibody
See all GNL1 primary antibodies
DescriptionRabbit polyclonal to GNL1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Orangutan
Synthetic peptide within Human GNL1 aa 50-100. The exact sequence is proprietary. NP_005266.2
Database link: P36915
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245634 was affinity purified using an epitope specific to GNL1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
FunctionPossible regulatory or functional link with the histocompatibility cluster.
Sequence similaritiesBelongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain.
DomainIn contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Gnl1 antibody
- GNL1_HUMAN antibody
- GTP binding protein HSR1 antibody
All lanes : Anti-GNL1 antibody (ab245634) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes
GNL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245634 at 10 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245634 at 0.4 µg/ml.
Lane 1: ab245634 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab245634 has not yet been referenced specifically in any publications.