Overview

  • Product name

  • Description

    Rabbit polyclonal to GNMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide corresponding toa region within N terminal amino acids 12-61 (GVAAEGLPDQ YADGEAARVW QLYIGDTRSR TAEYKAWLLG LLRQHGCHRV) of Mouse GNMT (NP_034451).

  • Positive control

    • Mouse small intestine lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
  • Tissue specificity

    Abundant in liver.
  • Involvement in disease

    Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.
  • Sequence similarities

    Belongs to the glycine N-methyltransferase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.1.1.20 antibody
    • Epididymis secretory sperm binding protein Li 182mP antibody
    • Glycine N methyltransferase antibody
    • Glycine N-methyltransferase antibody
    • Gnmt antibody
    • GNMT_HUMAN antibody
    • HEL S 182mP antibody
    • OTTHUMP00000016412 antibody
    see all

Images

  • Anti-GNMT antibody (ab118298) at 1 µg/ml + Mouse small intestine lysate at 10 µg

    Predicted band size: 33 kDa



    Gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

References

ab118298 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
10 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% Acrylamide resolving gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 07 2013

Answer

DISCOUNT CODE: XXXXXXX
Expiration date: 17-01-2013
Value: £235

I am very pleased to hear you would like to accept our offer and test ab118298 in Rat. This code will give you ab118298 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab118298 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting us.

The immunogen of antibody ab118298 is 98% similar with rat; though we have not tested the antibody with Rat samples however it is most likely to cross react.

https://www.abcam.com/gnmt-antibody-ab118298.html

If you are interested in using this antibody with Rat samples, please do not hesitate to contact us for 100% Abtrial discount code. You might get a your next antibody order free of charge with the Abtiral code. Please check terms and conditions at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up