Overview

  • Product name

  • Description

    Rabbit polyclonal to GNPAT/DAPAT
  • Host species

    Rabbit
  • Specificity

    ab75060 detects endogenous levels of total GNPAT/DAPAT protein.

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human GNPAT/DAPAT (C terminal).

  • Positive control

    • Extracts from HT-29 cells and RAW264.7cells
  • General notes

     This product was previously labelled as GNPAT

     

Properties

Applications

Our Abpromise guarantee covers the use of ab75060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
ELISA 1/20000.

Target

  • Pathway

    Membrane lipid metabolism; glycerophospholipid metabolism.
  • Involvement in disease

    Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2) [MIM:222765]. RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation.
  • Sequence similarities

    Belongs to the GPAT/DAPAT family.
  • Domain

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • Cellular localization

    Peroxisome membrane. Exclusively localized to the lumenal side of the peroxisomal membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acyl CoA dihydroxyacetonephosphateacyltransferase antibody
    • Acyl-CoA:dihydroxyacetonephosphateacyltransferase antibody
    • DAP AT antibody
    • DAP-AT antibody
    • DAPAT antibody
    • DHAP AT antibody
    • DHAP-AT antibody
    • DHAPAT antibody
    • Dihydroxyacetone phosphate acyltransferase antibody
    • EC 2.3.1.42 antibody
    • Glycerone-phosphate O-acyltransferase antibody
    • Glyceronephosphate O acyltransferase antibody
    • GNPAT antibody
    • GNPAT_HUMAN antibody
    • OTTHUMP00000036147 antibody
    see all

Images

  • All lanes : Anti-GNPAT/DAPAT antibody (ab75060) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from RAW264.7cells
    Lane 3 : extracts from HT-29 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 77 kDa
    Observed band size: 77 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Hossain MS  et al. Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling. PLoS One 11:e0150846 (2016). WB . Read more (PubMed: 26934370) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
10 µg
Specification
C2C12
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2011

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