Key features and details
- Rabbit polyclonal to GNPDA1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GNPDA1 antibody
See all GNPDA1 primary antibodies
DescriptionRabbit polyclonal to GNPDA1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Hamster, Cow, Orangutan
- IHC-P: Human testis tissue. WB: RT4 cell lysate. ICC/IF: U-251 MG cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab253022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSeems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo.
Sequence similaritiesBelongs to the glucosamine/galactosamine-6-phosphate isomerase family.
- Information by UniProt
- EC 126.96.36.199 antibody
- GlcN6P deaminase 1 antibody
- Glucosamine 6 phosphate deaminase 1 antibody
PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for GNPDA1 (green) using ab253022 at 4 µg/ml in ICC/IF.
Paraffin-embedded human testis tissue stained for GNPDA1 using ab253022 at 1/200 dilution in immunohistochemical analysis.
Anti-GNPDA1 antibody (ab253022) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253022 has not yet been referenced specifically in any publications.