Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab16216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use a concentration of 1 µg/ml.

Target

  • Relevance
    Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • GnRH associated peptide 1 antibody
    • GNRH1 antibody
    • Gonadotrophin releasing hormone 1 antibody
    • GRH antibody
    • HH12 antibody
    • LHRH antibody
    • LNRH antibody
    • Luliberin I antibody
    • Luteinizing releasing hormone antibody
    • Lutenizing hormome releasing hormone antibody
    • Progonadoliberin 1 antibody
    • Progonadoliberin I antibody
    • Prolactin release inhibiting factor antibody
    see all

References

ab16216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Recombinant protein (mixed with LbT2 cells 10ug lysate)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
0.054 µg
Specification
mixed with LbT2 cells 10ug lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Lilach Polinsky

Verified customer

Submitted Oct 10 2018

Answer

Thank you for contacting us. Both the ab5617 and ab16216 are raised against an immunogen synthetic peptide of HWSYGLRPG corresponding to amino acids 22-31 of GnRH. Comparing this to the corresponding sequence of Chicken GnRH (Swiss-Prot number P37042) reveals 8 of the 9 amino acids of the immunogen match exactly. We would therefore expect these antibodies to detect chicken GnRH. However, neither of these antibodies have been tested against chicken or with ELISA. Therefore, I can offer a discount off a future purchase if you buy either ab5617 or ab16216 now, test it in chicken with the ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test either ab5617 or ab16216 in chicken with the ELISA. I will then send a discount code. This code must be issued before purchasing ab5617 or ab16216 so please wait for my reply before ordering. 2. Purchase either ab5617 or ab16216 either by phone, fax, or online (www.abcam.com). 3. Test it in chicken with the ELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if either ab5617 or ab16216 turns out to be unsuitable for chicken with the ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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