Non-Abcam antibody was used: goat anti rabbit HRP conjugated Host species: Goat Clonality: Polyclonal Conjugation: HRP polymer
The LbT2 cell lysate not mixed with the recombinant protein
Specific: 47 kDa Non-specific: 15 kDa
The recombinant protein alone
GnRH lys6 was cross linked to a GFP tagged protein (471 amino acids). The protein is a chimeric protein, not yet published.
The sequence of the GnRH lys6 is : EHWSYKLRPG.
the cross linker is sulfo-GMBS, which cross links the lys6 of the GnRH to a cysteine in the chimeric protein.
The cross linking was verified by mass spec.
Lanes: 1) LbT2 cell lysate 10ug .
2) LbT2 cell lysate 10ug + 54ng of the GnRH lys6 cross linked protein.
3) LbT2 cell lysate 10ug + 5.4ng of the GnRH lys6 cross linked protein.
4) LbT2 cell lysate 10ug + 0.54ng of the GnRH lys6 cross linked protein.
5) 54ng of the GnRH lys6 cross linked protein
6) pre-stained marker lane.
Dr. Lilach Polinsky
Submitted Oct 10 2018
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