• Product name
  • Description
    Rabbit polyclonal to GnRH
  • Host species
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IHC-P, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human) conjugated to BSA.

  • Positive control
    • Hypothalamus
  • General notes
    LHRH is also known as Gonadotropin-releasing hormone (GNRH).



Our Abpromise guarantee covers the use of ab8491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    This antibody is suitable for use on paraffin embedded tissues without enzymatic antigen retrieval
    Advised working dilutions are 1:50-1:100.
    Dilution buffer is 0.15 M PBS with 1% Ovalbumin and 0.09% sodium azide.
    Optimal dilution should be tested by serial dilution.
  • Target

    • Relevance
      Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
    • Cellular localization
    • Database links
    • Alternative names
      • GnRH associated peptide 1 antibody
      • GNRH1 antibody
      • Gonadotrophin releasing hormone 1 antibody
      • GRH antibody
      • HH12 antibody
      • LHRH antibody
      • LNRH antibody
      • Luliberin I antibody
      • Luteinizing releasing hormone antibody
      • Lutenizing hormome releasing hormone antibody
      • Progonadoliberin 1 antibody
      • Progonadoliberin I antibody
      • Prolactin release inhibiting factor antibody
      see all


    ab8491 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    Thank you for your enquiry and your detailed protocol. We are very sorry to hear that you are having problem with this antibody. We have sold several vials of this antibody and do not have any problem with it. According to the datasheet, this product has been tested and characterised on human. Other species have not tested so we do not have any information if the antibody works in mouse. 1. We would recommend using human brain section as positive control. 2. As we understand, you fixed the samples in 4% PFA fix 3 hours but have not applied any antigen retrieval step. Formalin fixed tissue requires an antigen retrieval step before immunohistochemical staining can proceed. This is due to the formation of methylene bridges during fixation, which cross link proteins and therefore mask antigenic sites. 3. We would also recommend using 1% BSA as blocker rather than milk. 4. Your detection system is HRP-DAB. Have you tried to block endogenous peroxidase activity in the samples? We hope that this will help to solve the problem.

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    Unfortunately the epitope is not known. We know that it recognizes several epitopes on LH-RH. In the following reference there is data about the preparation of the antigen conjugate. Van Rees, et al., 1984, Acta Endocrinologica 104: 272-278

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    I have been unable to obtain a protocol for the use of this antibody in IHC from the originator. Furthermore there are no published references, so all the information we have is on the datasheet where we advise that use in IHC on PES should be without antigen retrieval and provide suggested started dilutions and a recommended dilution buffer. However, I can guarantee that if the antibody does not work as specified on the datasheet, we will provide an alternative product or offer a complete refund.

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