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RecombinantRabMAb

Recombinant Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
  • Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24529-70] to GnRH - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-GnRH antibody [EPR24529-70] - BSA and Azide free
    See all GnRH primary antibodies

  • Description

    Rabbit monoclonal [EPR24529-70] to GnRH - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Data from recombinant overexpression of GnRH demonstrating reactivity in WB, ICC, IP and Flow Cyt (intracelluar) is available.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse hypothalamus, Rat hypothalamus, HEK-293T transfected with Progonadoliberin-1 expression vector containing a myc-His-tag®. IHC-Fr: Mouse hypothalamus, Rat hypothalamus.

  • General notes

    ab284859 is the carrier-free version of ab281844

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab284859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Target

  • Relevance

    Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle. Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • GnRH associated peptide 1 antibody
      • GNRH1 antibody
      • Gonadotrophin releasing hormone 1 antibody
      • GRH antibody
      • HH12 antibody
      • LHRH antibody
      • LNRH antibody
      • Luliberin I antibody
      • Luteinizing releasing hormone antibody
      • Lutenizing hormome releasing hormone antibody
      • Progonadoliberin 1 antibody
      • Progonadoliberin I antibody
      • Prolactin release inhibiting factor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Progonadoliberin-1 expression vector containing a myc-His-tag® (Panel B) HEK-293T transfected with empty plasmid. tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the arcuate hypothalamic nucleus arc of rat hypothalamus. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the arcuate hypothalamic nucleus arc of mouse hypothalamus (PMID:19809474). The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hypothalamus tissue labelling GnRH with ab281844 at 150 (7.54 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on arcuate hypothalamic nucleus arc of mouse hypothalamus (arrow) is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the arcuate hypothalamic nucleus arc of rat hypothalamus. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Frozen sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labelling GnRH with ab281844 at 150 (7.54 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on arcuate hypothalamic nucleus arc of rat hypothalamus (arrow) is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      This data was developed using ab281844, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling GnRH with ab281844 at 1/600 (0.628 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat liver. The section was incubated with ab281844 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)
      Anti-GnRH antibody [EPR24529-70] - BSA and Azide free (ab284859)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab284859? Please let us know so that we can cite the reference in this datasheet.

    ab284859 has not yet been referenced specifically in any publications.

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