Product nameAnti-GnRHR antibody [A9E4]
See all GnRHR primary antibodies
DescriptionMouse monoclonal [A9E4] to GnRHR
SpecificityIt is unknown if ab24095 reacts with the receptor form 1 or 2.
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Pig
- IHC-Fr: human anterior pituitary sections WB/FACS: GT7-1 cells (hypothalamus cell line)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab24095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/20 - 1/40.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/20 - 1/40. Predicted molecular weight: 38 kDa.|
- Information by UniProt
- GnRH receptor antibody
- GnRH-R antibody
- GNRHR antibody
Overlay histogram showing MCF7 cells stained with ab24095 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24095, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
This product has been referenced in:
- Xu B et al. Increased Uterine NK cell numbers and perforin expression during the implantation phase in IVF Cycles with GnRH Antagonist Protocol. Sci Rep 7:39912 (2017). IHC . Read more (PubMed: 28045093) »
- Seitz S et al. Triple negative breast cancers express receptors for LHRH and are potential therapeutic targets for cytotoxic LHRH-analogs, AEZS 108 and AEZS 125. BMC Cancer 14:847 (2014). IHC ; Human . Read more (PubMed: 25410881) »