Overview

  • Product name

  • Description

    Rabbit polyclonal to GnRHR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide within Human GnRHR aa 62-75. The exact sequence is proprietary. NP_000397.
    Sequence:

    KWTQKKEKGKKLSR


    Database link: P30968

  • Positive control

    • 231 cell lysate, mouse testis tissue lysate and Human esophagus cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 38 kDa.
IHC-P 1/117 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-GnRHR antibody (ab183079) at 1/2350 dilution

    Lane 1 : 231 cell lysate
    Lane 2 : mouse testis tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 25 seconds
  • Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling GnRHR with ab183079 at 1/117 dilution. The right image is treated with the immunizing peptide.

References

This product has been referenced in:

  • Huang S  et al. Synthesis and Evaluation of 18F-Labeled Peptide for Gonadotropin-Releasing Hormone Receptor Imaging. Contrast Media Mol Imaging 2019:5635269 (2019). Read more (PubMed: 30983920) »
  • Zhang N  et al. Goserelin promotes the apoptosis of epithelial ovarian cancer cells by upregulating forkhead box O1 through the PI3K/AKT signaling pathway. Oncol Rep 39:1034-1042 (2018). Read more (PubMed: 29286125) »
See all 7 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Neutrophil cells)
Gel Running Conditions
Reduced Denaturing (12% SDS page gel)
Loading amount
30 µg
Treatment
5ug/ml GnRH agonist
Specification
Mouse Neutrophil cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 19 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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