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Tags & Cell Markers Subcellular Markers Organelles Lysosome
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-GNS antibody [EPR8329(2)] (ab154177)

  • Datasheet
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Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)
  • Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)
  • Anti-GNS antibody [EPR8329(2)] (ab154177)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8329(2)] to GNS
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Protein
Recombinant Human GNS protein (ab151650)
Knockout
Product image
Human GNS knockout HeLa cell line (ab265495)

View more associated products

Overview

  • Product name

    Anti-GNS antibody [EPR8329(2)]
    See all GNS primary antibodies
  • Description

    Rabbit monoclonal [EPR8329(2)] to GNS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to residues in Human GNS (UniProt: P15586).

  • Positive control

    • WB: HeLa, PC3, NCI-H460 and 293T cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8329(2)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Alternative Versions

    • Anti-GNS antibody [EPR8329(2)] - BSA and Azide free (ab249077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human GNS knockout HeLa cell line (ab265495)
  • KO cell lysates

    • Human GNS knockout HeLa cell lysate (ab257975)
  • Recombinant Protein

    • Recombinant Human GNS protein (ab151650)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 62 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Involvement in disease

      Defects in GNS are the cause of mucopolysaccharidosis type 3D (MPS3D) [MIM:252940]; also known as Sanfilippo D syndrome. MPS3D is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
    • Sequence similarities

      Belongs to the sulfatase family.
    • Post-translational
      modifications

      The form A (78 kDa) is processed by internal peptidase cleavage to a 32 kDa N-terminal species (form B) and a 48 kDa C-terminal species.
      The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
    • Cellular localization

      Lysosome.
    • Target information above from: UniProt accession P15586 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2799 Human
      • Omim: 607664 Human
      • SwissProt: P15586 Human
      • Unigene: 334534 Human
      • Alternative names

        • 2610016K11Rik antibody
        • AU042285 antibody
        • C87209 antibody
        • G6S antibody
        • Glucosamine (N-acetyl) 6 sulfatase antibody
        • Glucosamine 6 sulfatase antibody
        • Glucosamine-6-sulfatase antibody
        • GNS antibody
        • GNS_HUMAN antibody
        • MGC21274 antibody
        • N acetylglucosamine 6 sulfatase [Precursor] antibody
        • N-acetylglucosamine-6-sulfatase antibody
        • N28088 antibody
        see all

      Images

      • Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)
        Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)
        All lanes : Anti-GNS antibody [EPR8329(2)] (ab154177) at 1/1000 dilution

        Lane 1 : Wild-type HeLa cell lysate
        Lane 2 : GNS knockout HeLa cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

        Predicted band size: 62 kDa
        Observed band size: 90 kDa
        why is the actual band size different from the predicted?



        Lanes 1-3: Merged signal (red and green). Green - ab154177 observed at 90 kDa. Red - loading control ab7291 observed at 50 kDa.

        ab154177 Anti-GNS antibody [EPR8329(2)] was shown to specifically react with GNS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265495 (knockout cell lysate ab257975) was used. Wild-type and GNS knockout samples were subjected to SDS-PAGE. ab154177 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

         

      • Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)
        Western blot - Anti-GNS antibody [EPR8329(2)] (ab154177)
        All lanes : Anti-GNS antibody [EPR8329(2)] (ab154177) at 1/10000 dilution

        Lane 1 : PC3 cell lysate
        Lane 2 : NCI-H460 cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 62 kDa

      • Anti-GNS antibody [EPR8329(2)] (ab154177)
        Anti-GNS antibody [EPR8329(2)] (ab154177)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab154177? Please let us know so that we can cite the reference in this datasheet.

      ab154177 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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