Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)
Key features and details
- Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, Flow Cyt, IHC-P, ELISA, ICC
Overview
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Product name
Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Armenian hamster -
Specificity
This antibody is specific to Armenian Hamster IgG -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, IHC-P, ELISA, ICCmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
|
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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|
| ICC |
1/200 - 1/1000.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
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IHC-P
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC
1/200 - 1/1000. |
Images
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Immunocytochemistry - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)
ICC/IF image of ab243248 stained Neuro2a cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab243248, 1.6µg/ml) overnight at +4°C. The secondary antibody (green) was ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Immunocytochemistry - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)ICC/IF image of ab80952 stained MCF-7 cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab80952, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab173003 Alexa Fluor 488 Goat polyclonal Secondary Antibody to Armenian Hamster IgG - (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (6)
ab173003 has been referenced in 6 publications.
- Stavoe AK et al. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. Elife 8:N/A (2019). PubMed: 31309927
- Liu X et al. Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities. Immunity 50:317-333.e6 (2019). PubMed: 30683620
- Ohyama K & Suzuki K Dihydrocapsiate improved age-associated impairments in mice by increasing energy expenditure. Am J Physiol Endocrinol Metab 313:E586-E597 (2017). PubMed: 28811294
- Cheng M et al. Commensal microbiota maintains alveolar macrophages with a low level of CCL24 production to generate anti-metastatic tumor activity. Sci Rep 7:7471 (2017). PubMed: 28785009
- Zhu H et al. RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease. EMBO Mol Med 9:589-604 (2017). IHC-Fr . PubMed: 28377495
- Hongu T et al. Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial ß1 integrin recycling. Nat Commun 6:7925 (2015). ICC/IF . PubMed: 26239146
