Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameGoat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)
See all IgG secondary antibodies -
Host speciesGoat
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Target speciesArmenian hamster
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SpecificityThis antibody is specific to Armenian Hamster IgG
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
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Immunogen
The details of the immunogen for this antibody are not available.
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ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab173003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| Flow Cyt | 1/2000. | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)ICC/IF image of ab80952 stained MCF-7 cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab80952, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab173003 Alexa Fluor 488 Goat polyclonal Secondary Antibody to Armenian Hamster IgG - (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (ab173003)
References
This product has been referenced in:
- Cheng M et al. Commensal microbiota maintains alveolar macrophages with a low level of CCL24 production to generate anti-metastatic tumor activity. Sci Rep 7:7471 (2017). Read more (PubMed: 28785009) »
- Zhu H et al. RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease. EMBO Mol Med 9:589-604 (2017). IHC-Fr . Read more (PubMed: 28377495) »
