Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase) (ab5746)

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Overview

  • Product name
    Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Armenian hamster
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • Immunogen

    Armenian Hamster IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
WB 1/500 - 1/2500.
ELISA 1/2000 - 1/25000.
IHC Use at an assay dependent dilution.

References

ab5746 has not yet been referenced specifically in any publications.

Publishing research using ab5746? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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do you have any image staining with this secondary for IHC with BCIP/NBT?

Thank you for contacting us.
This secondary antibody can be used for IHC, and the staining detected using the NBT/BCIP substrate ab7468. Although we do not have an image using this specific secondary on file, I have attached an image of AP conjuga...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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