Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase) (ab5746)

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Key features and details

  • Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Biotin FITC Texas Red ® TRITC Unconjugated

Overview

  • Product name

    Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Armenian hamster

  • Specificity

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum.

  • Tested applications

    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details

  • Immunogen

    Armenian Hamster IgG whole molecule

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.

  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent dilution.
WB
1/500 - 1/2500.
ELISA
1/2000 - 1/25000.
IHC
Use at an assay dependent dilution.
Notes
ICC/IF
1/200 - 1/1000.
Dot blot
Use at an assay dependent dilution.
WB
1/500 - 1/2500.
ELISA
1/2000 - 1/25000.
IHC
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab5746? Please let us know so that we can cite the reference in this datasheet.

ab5746 has been referenced in 1 publication.

  • Zhou N  et al. Matrine-induced apoptosis in Hep3B cells via the inhibition of MDM2. Mol Med Rep 15:442-450 (2017). PubMed: 27959389

Customer reviews and Q&As

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Question

do you have any image staining with this secondary for IHC with BCIP/NBT?

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Answer

Thank you for contacting us.
This secondary antibody can be used for IHC, and the staining detected using the NBT/BCIP substrate ab7468. Although we do not have an image using this specific secondary on file, I have attached an image of AP conjugated secondary with NBT/BCIP (left image, negative control, right stained V. harveyi 639 cells).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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