Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase) (ab5746)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Armenian hamster -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum. -
Tested applications
Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details -
Immunogen
Armenian Hamster IgG whole molecule
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose. -
Conjugation notes
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
Our Abpromise guarantee covers the use of ab5746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200 - 1/1000. | |
| Dot blot | Use at an assay dependent dilution. | |
| WB | 1/500 - 1/2500. | |
| ELISA | 1/2000 - 1/25000. | |
| IHC | Use at an assay dependent dilution. |
References
This product has been referenced in:
- Zhou N et al. Matrine-induced apoptosis in Hep3B cells via the inhibition of MDM2. Mol Med Rep 15:442-450 (2017). Read more (PubMed: 27959389) »
