Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase) (ab5746)

Overview

  • Product name
    Goat Anti-Armenian hamster IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Armenian hamster
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian Hamster and Armenian Hamster Serum.
  • Tested applications
    Suitable for: ICC/IF, Dot blot, WB, ELISA, IHCmore details
  • Immunogen

    Armenian Hamster IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Dot blot Use at an assay dependent dilution.
WB 1/500 - 1/2500.
ELISA 1/2000 - 1/25000.
IHC Use at an assay dependent dilution.

References

ab5746 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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This secondary antibody can be used for IHC, and the staining detected using the NBT/BCIP substrate ab7468. Although we do not have an image using this specific secondary on file, I have attached an image of AP conjuga...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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