Overview

  • Product name
    Goat Anti-Armenian hamster IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Host species
    Goat
  • Target species
    Armenian hamster
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Cross reactivity against mouse has not been determined.
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blot, ELISA, Immunomicroscopy, IHC-Pmore details
  • Immunogen

    Armenian Hamster IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ELISA 1/250000.
Immunomicroscopy Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Images

  • Ab5744 was used at dilution 1/500 with the primary antibody ab33483 in IHC-P. See the review on ab33483.

References

This product has been referenced in:
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

The appropriate secondary antibody for use with this product will be a Secondary Antibody to Armenian Hamster IgG such as ab5744.

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Answer

Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would like to offer a second option which will allow you to perform these experiments but will allow you future flexibility as well. I recommend purchase of unlabeled secondary antibodies and labeling these with the marker of your choice (biotin, fluorochrome) using our EasyLink direct conjugation kits. In this way you will be able to perform serial stains using a single primary/secondary combination and will be able to optimize and trust them in this assay. You will also have the ability to use these same antibodies, apply different labels and use them in different assays such as a double or triple staining with fluorescent tags, western blot with HRP or other assays. Our EasyLink antibody conjugation kits provide asimple and quick linking process whichis part of a new generation of conjugation procedures. These eliminate many of the difficulties which were common in previous generation of conjugation kits andallow a quick and easy process to conjugate an antibody of your choice, in whatever function you need. It has the advantage of being aone-stepantibody labeling method withno separation steps. Further information about our EasyLink kits may be found at the following links: Landing Page:https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink FAQs:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 The unconjugated antibodies which I would recommend would be: ab98800, anti-mouse, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-pre-adsorbed-ab98800.html ab98383, anti-rat, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-pre-adsorbed-ab98383.html ab5738, anti-Armenian hamster, unconjugated:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5738.html For your initial biotin labeling I would recommend one of the following EasyLink Biotin (type A) labeling kits: 3 x10ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10%C2%B5g-Biotin-ab102865.html 3 x 100ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100%C2%B5g-Biotin-ab102864.html The pre-conjugated biotin labeled antibodies can be found at the following links: ab98802 anti-mouse, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-pre-adsorbed-ab98802.html ab98414 anti-rat, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-Biotin-pre-adsorbed-ab98414.html ab5744 anti-Armenian hamster, biotin:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5744.html Finally, as the anti-mannose receptor antibody has not yet been tested in IHC-P, I would like to offer to you enrollment in our 100% Abreview testing discount program. DISCOUNT CODE: XXXXXXX Expiration date: XXXXX There is no obligation for you to participate in this program; however should you wish to participate this code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P with ab8918 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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Answer

Thank you for this information.

I have noted that this primary/secondary antibody combination has been used and reviewed by another customer. Their Abreview includes a detailed protocol which you may wish to review. This review is at:https://www.abcam.com/index.html?datasheet=33483&tab=abreviews&intabreviewid=14724

It may be in your case that much of the background is caused by secondary antibody binding to
endogenous mouse IgG in the tissue being stained, and to Fc receptors on B cells, plasma cells and macrophages. As such it may be valuable to use some of the staining procedures for mouse on mouse staining. Blocking endogenous IgG may be done by incubating the sections with an unconjugated Fab fragment likeGoat F (ab')2 polyclonal Secondary Antibody to Mouse IgG - (Fab')2, pre-adsorbed (ab98754),after the normal blocking step.More details may be found at this link:
https://www.abcam.com/index.html?pageconfig=resource&rid=11465

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for contacting us.

This product is not tested against or cross adsorbed against mouse. Since this is not a cross adsorbed secondary antibody, cross reactivity with other species may often be expected.

I have searched our catalog for other anti-Armenian Hamster IgG product which are cross absorbed by we currently do not offer any of these. I would very much like to help you to resolve this problem however. If you could please send me the details of your protocol I would be glad to review those and see if I might be able to make suggestions which will reduce background reactivity. Please include as much information as posibble in those notes.


I look forward to hearing from you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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