Goat Anti-Armenian hamster IgG H&L (FITC) (ab5739)
Key features and details
- Goat Anti-Armenian hamster IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, ELISA, Flow Cyt, Immunomicroscopy
Related conjugates and formulations
Overview
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Product name
Goat Anti-Armenian hamster IgG H&L (FITC)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Armenian hamster -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. -
Tested applications
Suitable for: ICC/IF, IHC-P, ELISA, Flow Cyt, Immunomicroscopymore details -
Immunogen
Armenian Hamster IgG whole molecule
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Conjugation
FITC. Ex: 493nm, Em: 528nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
BSA is protease free -
Concentration information loading...
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Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads. -
Conjugation notes
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons). Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.3 moles FITC per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration. PubMed: 24146823
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ELISA |
1/10000 - 1/50000.
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Flow Cyt |
1/500 - 1/2500.
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Immunomicroscopy |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. PubMed: 24146823 |
ELISA
1/10000 - 1/50000. |
Flow Cyt
1/500 - 1/2500. |
Immunomicroscopy
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (6)
ab5739 has been referenced in 6 publications.
- Liang C et al. Neuroprotection by Nrf2 via modulating microglial phenotype and phagocytosis after intracerebral hemorrhage. Heliyon 9:e13777 (2023). PubMed: 36852060
- Flanagan SP et al. Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers. Anal Methods 13:1191-1203 (2021). PubMed: 33605950
- Ma F et al. The requirement of CD8+ T cells to initiate and augment acute cardiac inflammatory response to high blood pressure. J Immunol 192:3365-73 (2014). ICC/IF ; Mouse . PubMed: 24600037
- Sato A et al. Inhibition of plasmin attenuates murine acute graft-versus-host disease mortality by suppressing the matrix metalloproteinase-9-dependent inflammatory cytokine storm and effector cell trafficking. Leukemia N/A:N/A (2014). PubMed: 24791857
- Luger D et al. Expression of the B-Cell Receptor Component CD79a on Immature Myeloid Cells Contributes to Their Tumor Promoting Effects. PLoS One 8:e76115 (2013). IHC-P . PubMed: 24146823
- Gramoun A et al. Fibronectin inhibits osteoclastogenesis while enhancing osteoclast activity via nitric oxide and interleukin-1ß-mediated signaling pathways. J Cell Biochem 111:1020-34 (2010). Flow Cyt . PubMed: 20672308