Key features and details
- Goat Anti-Bird IgY H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-P, ICC/IF
Product nameGoat Anti-Bird IgY H&L
See all IgY secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA ab112771 reacts specifically with Duck, Sparrow, Dove and Chicken IgG. Turkey, Ostrich, Emu, Quail, Macaw and Cockateil IgG are also detected. No antibody was detected against non-immunoglobulin serum proteins. ab112771 may cross react with IgG from other species.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Other Immunogen Type corresponding to IgY.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesGoats were immunized with purified IgG from dove, duck, sparrow and chicken serum. ab112771 was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab112771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. colorimetric detection; 1/1000 - 1/30000 chemiluminescent detection.|
|ELISA||1/100 - 1/500. for coating; 1/1000 - 1/30000 as a primary.|
|IHC-P||1/200 - 1/2000.|
|ICC/IF||1/200 - 1/2000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab112771 has been referenced in 1 publication.
- Liu M et al. Roles of B739_1343 in iron acquisition and pathogenesis in Riemerella anatipestifer CH-1 and evaluation of the RA-CH-1?B739_1343 mutant as an attenuated vaccine. PLoS One 13:e0197310 (2018). PubMed: 29847566