Overview

  • Product name

    Goat Anti-Bird IgY H&L (HRP)
    See all IgY secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Bird IgY - H&L (HRP)
  • Host species

    Goat
  • Target species

    Bird
  • Specificity

    By immunoelectrophoresis and ELISA ab112773 reacts with Dove, Duck, Sparrow, Chicken, Turkey, Emu, Ostrich, Quail, Macaw and Cockateil IgG. No antibody was detected against nonimmunoglobulin serum proteins. May cross react with IgG from other species.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Immunogen

    Other Immunogen Type corresponding to IgY.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Goats were immunized with purified IgG from Dove, Duck, Sparrow and Chicken serum. ab112773 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
  • Conjugation notes

    Molar enzyme/antibody protein ratio is 4:1.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. colorimetric detection; 1/1000 - 1/30000 chemiluminescent detection.
ELISA 1/10000 - 1/100000. as a primary.
IHC-P 1/200 - 1/500.
ICC/IF 1/200 - 1/500.

References

This product has been referenced in:

  • Huang J  et al. Oral Vaccination with a DNA Vaccine Encoding Capsid Protein of Duck Tembusu Virus Induces Protection Immunity. Viruses 10:N/A (2018). ELISA . Read more (PubMed: 29642401) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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