Overview

  • Product name

    Goat Anti-Chicken IgY H&L (6nm Gold)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: Electron Microscopymore details
  • Immunogen

    Chicken Ig whole molecule.

  • Conjugation

    Gold 6nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions. The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution. The method of production of this product allows the small particle size of 6nm to be used instead of 5nm with the same sensitivity but increased visibility in the standard transmission electron microscope (See also van Bergen en Henegouwen & Leunissen, Histochemistry, 85, (1986), 81.)

    Light Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    This product has been referenced in:

    • Stanton RJ  et al. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. J Clin Invest 120:3191-208 (2010). Electron Microscopy . Read more (PubMed: 20679731) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your reply. I am sorry we missed eachother yesterday.


    I'm pleased to help answer your questions:

    1. Icanconfirm weregrettablydo not currently have a goat anti-mouse IgG (H+L) gold conjugated and pre-adsorbed in the catalog. I am sorry we do not have a product to suggest thatmeets your requirements on this occasion.

    2. I am sorry I am not an expert in EM and am waiting for more information from the laboratories in order to provide an answer to your second question for you. It seems in your experience it is possible to tell the difference between the different sizes of gold particle, and I can imagine this is possible to do with EM imaging. However, I would like to confirm this. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done in order to test these various antibodies together to see if they are distinguishable.


    As soon aswe have investigated this case with the originator of ab105265, I will contact you again as soon as possible.I understand your concerns and appreciate your patience.

    Read More

    Answer

    Thank you once again for your recent enquiries.

    Following on from my previous email, I can now confirm that ab39604 Goat polyclonal Secondary Antibody to Chicken IgYis cross adsorbed against human, mouse, rabbit, rat and sheep serum proteins.

    I hope this information will be helpful to you. I look forward to hearing from you with the further details regarding ab105265.

    Read More

    Answer

    Thankyou for your telephone call yesterday afternoon. I am sorry to hear you have some concerns regardingthe results in TEM from ab105265.

    I would like to reassure you that this antibody is tested and covered by our guarantee forEM and human. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality records. As discussed on the telephone, in order to proceed with this, I would appreciate if you are able to provide more information:

    1. The order number and date of purchase

    2. Please provide the images and data that indicate the gold is 1 - 2 nm

    3. Please provide information on the sample and procedure you are using.


    With regards to your other questions:

    Do you have a goat anti chicken IgY, 6 nm gold? Preabsorbed?

    Yes, ab39604 is cross absorbed according to the datasheet. I am currently contacting our source for further information and to confirm this.

    We need a goat anti-chicken IgY conjugated to a fluorophore with excitation 555nm 643 nm?

    We do have many fluorescently conjugatedcross absorbedgoat anti-chicken IgG secondaries available. I am sorry, these are not conjugated to fluorophores with excitation of 555nm 643 nm.However, I am sending the search results from our webpages, andI hope perhaps this may still be useful:

    https://www.abcam.com/index.html?pageconfig=searchresults&startPage=1

    Thank you for your time and cooperation. We look forward to receiving therequested information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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