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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab182019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/20000 - 1/200000.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Cross-reactivity of the polyclonal secondary antibody ab182019 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1µg/ml (50µl/well) amd incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182019 was then added starting at 1 µg/ml and gradually diluted 1:4 (50µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1:20,000 dilution (50µl/well), followed by incubation for 1h at RT.
For the batch tested, ab182019 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG and rabbit IgG.
ab182019 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"