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Secondary antibodies anti-Chicken IgY Fluorophore Alexa Fluor® 488
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (72)

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
  • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

Key features and details

  • Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

You may also be interested in

Primary
Product image
Anti-GFP antibody (ab13970)
Blocking
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Normal Goat Serum (ab7481)

View more associated products

Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)
    See all IgY secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 488)
  • Host species

    Goat
  • Target species

    Chicken
  • Specificity

    This antibody is specific to Chicken IgY
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Fluorophore
    • Alexa Fluor® 488

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab150169 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr (1)
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    Use at an assay dependent concentration.
    IHC-P (1)
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    1/2000.

    Images

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

      ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150169 Alexa Fluor® 488 goat anti-chicken IgY (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

    • Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
      Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (72)

    Publishing research using ab150169? Please let us know so that we can cite the reference in this datasheet.

    ab150169 has been referenced in 72 publications.

    • Taglialegna A  et al. The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers. NPJ Biofilms Microbiomes 6:15 (2020). PubMed: 32221298
    • Kur IM  et al. Neuronal activity triggers uptake of hematopoietic extracellular vesicles in vivo. PLoS Biol 18:e3000643 (2020). PubMed: 32176686
    • Meng Q  et al. Protective effects of histone deacetylase inhibition by Scriptaid on brain injury in neonatal rat models of cerebral ischemia and hypoxia. Int J Clin Exp Pathol 13:179-191 (2020). PubMed: 32211098
    • Wang Z  et al. Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors. Nat Commun 11:2163 (2020). PubMed: 32358530
    • Kitchen P  et al. Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema. Cell 181:784-799.e19 (2020). PubMed: 32413299
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    I am looking for a secondary antibody for Anti-GFP antibody (ab13970) (made in chicken).

    Would this be an appropriate secondary antibody?

    Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)

    (I’m wondering if the anti-chicken IgG is better)

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2014

    Answer



    I am happy to confirm that ab150169 (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)) is a suitable secondary antibody for ab13970 (Anti-GFP antibody (ab13970)).

    I would like to note that chicken IgY and chicken IgG are the same kind of immunoglobulin.

    The nomenclature sometimes is not very clear as IgY is often mislabeled as Immunoglobulin G (IgG) in older literature, and sometimes in commercial product catalogs, due to its functional similarity to mammalian IgG

    Chicken IgY is the major circulating antibody found in chickens and is the avian counterpart to mammalian IgG’s. IgYs are equivalent to IgGs in most applications, including Western Blotting, ELISAs, immunohistochemistry, immunocytochemistry.

    IgYs have the same structure as a mammalian IgG, with 2 heavy (“nu” chains, ˜67-70 kDa) and 2 light chains (22-30 kDa)

    Unlike the gamma-chains of IgG, however, the heavy chains of IgYs lack Fc domains, which means that IgYs do not fix complement nor bind protein A or protein G.
    Chickens also produce an alternatively-spliced form of the heavy chain in which the Cnu3 and Cnu4 domains are deleted. In some individual animals, this F(ab)2-like heavy chain can be seen running at 40 kDa in SDS-(10%) polyacrylamide gels.

    More information can also be found here:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11258&pid=11287

    Read More

    Anja Hoffmann

    Abcam Scientific Support

    Answered on Dec 10 2014

    primary: chicken anti GFAP (ab4674) secondary: ab150169

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    1:500 dilution in Blocking solution (10% NGS, 0,3% TritonX100)

    Incubation time: 1h

    Good staining results!

    Abcam user community

    Verified customer

    Submitted Jun 22 2020

    Goat anti Chicken AF488

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Dilution secondary AB: 1:250
    Dilution Buffer: Normal Serum

    Abcam user community

    Verified customer

    Submitted Jun 10 2020

    ICC of Mouse brain organotypic slices

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Secondary anti-body worked well and gave strong clear staining in my samples

    Ms. Kirsty Ireland

    Verified customer

    Submitted Mar 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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