Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Overview
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Product name
Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)
See all IgY secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 488) -
Host species
Goat -
Target species
Chicken -
Specificity
This antibody is specific to Chicken IgY -
Tested applications
Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
Our Abpromise guarantee covers the use of ab150169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | 1/2000. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150169 Alexa Fluor® 488 goat anti-chicken IgY (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab150169 has been referenced in 42 publications.
- Lisieski MJ et al. Divergent effects of repeated cocaine and novel environment exposure on locus coeruleus c-fos expression and brain catecholamine concentrations in rats. Brain Behav 9:e01222 (2019). PubMed: 30790470
- Li YP et al. A TRIM71 binding long noncoding RNA Trincr1 represses FGF/ERK signaling in embryonic stem cells. Nat Commun 10:1368 (2019). PubMed: 30911006
- Kakava-Georgiadou N et al. An Intersectional Approach to Target Neural Circuits With Cell- and Projection-Type Specificity: Validation in the Mesolimbic Dopamine System. Front Mol Neurosci 12:49 (2019). PubMed: 30873002
- Spitzer SO et al. Oligodendrocyte Progenitor Cells Become Regionally Diverse and Heterogeneous with Age. Neuron 101:459-471.e5 (2019). PubMed: 30654924
- Musso PY et al. Closed-loop optogenetic activation of peripheral or central neurons modulates feeding in freely moving Drosophila. Elife 8:N/A (2019). PubMed: 31322499
