For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I am looking for a secondary antibody for Anti-GFP antibody (ab13970) (made in chicken).
Would this be an appropriate secondary antibody?
Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
(I’m wondering if the anti-chicken IgG is better)
Asked on Dec 10 2014
I am happy to confirm that ab150169 (Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)) is a suitable secondary antibody for ab13970 (Anti-GFP antibody (ab13970)).
I would like to note that chicken IgY and chicken IgG are the same kind of immunoglobulin.
The nomenclature sometimes is not very clear as IgY is often mislabeled as Immunoglobulin G (IgG) in older literature, and sometimes in commercial product catalogs, due to its functional similarity to mammalian IgG
Chicken IgY is the major circulating antibody found in chickens and is the avian counterpart to mammalian IgG’s. IgYs are equivalent to IgGs in most applications, including Western Blotting, ELISAs, immunohistochemistry, immunocytochemistry.
IgYs have the same structure as a mammalian IgG, with 2 heavy (“nu” chains, ˜67-70 kDa) and 2 light chains (22-30 kDa)
Unlike the gamma-chains of IgG, however, the heavy chains of IgYs lack Fc domains, which means that IgYs do not fix complement nor bind protein A or protein G.
Chickens also produce an alternatively-spliced form of the heavy chain in which the Cnu3 and Cnu4 domains are deleted. In some individual animals, this F(ab)2-like heavy chain can be seen running at 40 kDa in SDS-(10%) polyacrylamide gels.
More information can also be found here:
Anja HoffmannAbcam Scientific Support
Answered on Dec 10 2014