Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

Overview

  • Product name
    Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed
    See all IgY secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed
  • Host species
    Goat
  • Target species
    Chicken
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation
    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000.

Images

  • ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Darling TL  et al. Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging. Front Immunol 8:1197 (2017). Read more (PubMed: 29021793) »
  • Becerra SC  et al. An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue. BMC Res Notes 9:216 (2016). Read more (PubMed: 27071769) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry
Application:Immunocytochemistry
Sample: Species: Human
Blocking step:1% BSAin 1xTBS
Primary Antibody: Anti-200 kD Neurofilament Heavy antibody (ab4680), 2 hours Temperature: 4°C, Diluent: 1xTBS with 0.1% BSA
Secondary Antibody: ab150176, Dilution: 1/1000 Incubation time:1 hours at RT Diluent: 1xTBS with 0.1% BSA
Detection method: 594 excitation
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Submitted Feb 18 2016

Application
Immunocytochemistry
Application: ICC
Sample: Species: GFP plasmid overexpression
Blocking step:1X BSA
Primary Antibody: anti-GFP (ab13970), Dilution: 1/200 Incubation time:13 hours at 4°C Diluent: 1xTBS
Secondary Antibody: ab150176, Dilution: 1/400 Incubation time:2 hours at RT Diluent: 1xTBS
Detection method: 594 excitation
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Submitted Nov 02 2015

Abreviews
Application
Western blot
Application: Western blot

Sample: Species: Mouse Type: Cell lysate - whole cell

Loading amount: 25 µg, Specification: Cardiomyocytes overexpressed with GFP reporter plasmid

Gel Running Conditions: Reduced Denaturing (15% PAGE)

Blocking step: Milk as blocking agent for 1 hour at RT°C Blocking Concentration: 5%


Primary Antibody: anti-GFP (ab13970), Dilution: 1/2000 Incubation time:4 hours at 4°C Diluent: 1xTBS


Secondary Antibody: ab150176, Dilution: 1/5000 Incubation time:2 hours at RT Diluent: 1xTBS


Detection method: 594 excitation


Bands: Specific: ~25 kDa, Non-specific: none
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Submitted Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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