Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Alkaline Phosphatase)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, Dot blotmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 50% Glycerol, 0.0014% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads.
  • Conjugation notes

    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality

    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
ELISA 1/2000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/500 - 1/2500.
Dot blot Use at an assay dependent concentration.

References

ab6878 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your inquiry. I can confirm that both recognize whole chicken IgY and IgG (H+L). However, ab7119 has been cross adsorbed to remove reactivity with bovine, goat, guinea pig, hamster, horse, human, mouse, rabbit, rat and sheep serum proteins. Meanwhile, ab6878 has not been cross adsorbed. Therefore, I would suggest using ab7119 instead as it will have less cross reactivity with bovine. I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. Only product ab7119 has been tested against human serum using IEP and it showed no reaction with human serum. This is also the only one of the four that has been preadsorbed against human proteins. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Answer

Thanks for your email. On the phone you mentioned that you received 20 ul of antibody - I thought that you were talking about ab6878, but were you referring to ab14297?You should have received 50 ul in your vial of ab14297. Please let me know, if there wasn't 50 ul I will of course send you another vial free of charge. I understand your concern about pricing. We feel that our price is not just for the antibody, but also includes excellent customer and technical service. We also guarantee that our products will work as stated on the online datasheets and if not we will provide a replacement or refund. So please do let us know how the antibody works out for you.

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Question
Answer

Thank you for your enquiry. The concentration of the most recent lot is 0.54 mg/ml so there should be 1.85 ml in the vial. Can you please give me the lot number that you received (it is located on the vial) and the Abcam order number or purchase order number that was used? Thank you, and I look forward to hearing from you.

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