Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab7119)

Overview

  • Product name
    Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed
    See all IgY secondary antibodies
  • Host species
    Goat
  • Target species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native chicken IgY (purified).

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.00
    Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
ELISA
IHC-P
IHC-Fr
Immunomicroscopy
WB
  • Application notes
    Dot: Use at an assay dependant dilution.
    ELISA: 1/1000 - 1/4000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab7119 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your inquiry. I can confirm that both recognize whole chicken IgY and IgG (H+L). However, ab7119 has been cross adsorbed to remove reactivity with bovine, goat, guinea pig, hamster, horse, human, mouse, rabbit, rat and sheep serum proteins. Meanwhile, ab6878 has not been cross adsorbed. Therefore, I would suggest using ab7119 instead as it will have less cross reactivity with bovine. I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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