Overview

  • Product name
    Goat Anti-Chicken IgY H&L (Biotin)
    See all IgY secondary antibodies
  • Host species
    Goat
  • Target species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/3000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/3000 - 1/30000.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs

    ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in  TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Goat anti-Chicken IgY H&L (Biotin) secondary antibody (ab6876)
    Immunohistochemistry (Frozen sections) - Goat anti-Chicken IgY H&L (Biotin) secondary antibody (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.
    Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.

References

This product has been referenced in:
  • Wang L  et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). Read more (PubMed: 29670081) »
  • Huang B  et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . Read more (PubMed: 27688768) »

See all 5 Publications for this product

Publishing research using ab6876? Please let us know so that we can cite the reference in this datasheet.

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4 reviews or Q&A

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GFP expresssing Mouse astrocytes in brain

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Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin wax section was subjected to HIER before incubating in anti GFP ( ab13970). Section was then incubated with ab6876 ( 1/200) followed by stABCpx. GFP was visualised using DAB chromogen
abreview image
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 25 2013

I would like to know were is exacli attached the biotin. Is it only in the Fc region of the antibody?

I have been informed that amine reactive biotins were used to conjugate Lysine sites of antibodies. It is not only in the Fc region. Please do not hesitate to contact us again if you need more information,

I am thinking of using this antibody as a negative control for a biotinylated goat polyclonal Ab. Have you got any data regarding cross-reactions with human, rabbit and mouse proteins (especially Ig)?

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. At this time, we do not have any data about cross-reactivity with human, rabbit and mouse proteins.

Is this Antibody compartible (regarding sensivity) with Antirabbit IgG-Biotin from DAKO?

We don't have any data on the sensitivity of antibodies from DAKO, so its' difficult to compare. We do know that this antibody recognises 1µg chicken IgG at a working dilution of at least 1/20000.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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