Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameGoat Anti-Chicken IgY H&L (Biotin)
See all IgY secondary antibodies -
Host speciesGoat
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Target speciesChicken
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Tested applicationsSuitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
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Immunogen
chicken IgY whole molecule
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ConjugationBiotin
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.60
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
PurityIgY fraction
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Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Applications
Our Abpromise guarantee covers the use of ab6876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500 - 1/3000. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent dilution. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | 1/3000 - 1/30000. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Goat anti-Chicken IgY H&L (Biotin) secondary antibody (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.
References
This product has been referenced in:
- Wang L et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). Read more (PubMed: 29670081) »
- Huang B et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . Read more (PubMed: 27688768) »
