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Secondary antibodies anti-Chicken IgY Biotin
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Goat Anti-Chicken IgY H&L (Biotin) (ab6876)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)

Key features and details

  • Goat Anti-Chicken IgY H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgY
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Biotin)
    See all IgY secondary antibodies
  • Host species

    Goat
  • Target species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Biotin

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6876 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    1/500 - 1/3000.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent dilution.
    IHC-P (1)
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Immunomicroscopy
    Use at an assay dependent concentration.
    WB
    1/3000 - 1/30000.
    Notes
    ICC/IF
    1/500 - 1/3000.
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent dilution.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    Immunomicroscopy
    Use at an assay dependent concentration.
    WB
    1/3000 - 1/30000.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.

      ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in  TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.

    • Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
      Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.
      Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab6876? Please let us know so that we can cite the reference in this datasheet.

    ab6876 has been referenced in 6 publications.

    • Forbes D  et al. Liquid Dermal Scaffold With Adipose-Derived Stem Cells Improve Tissue Quality in a Murine Model of Impaired Wound Healing. J Burn Care Res 40:550-557 (2019). PubMed: 31188436
    • Wang L  et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). PubMed: 29670081
    • Huang B  et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . PubMed: 27688768
    • Huang B  et al. Differential expression of estrogen receptor a, ß1, and ß2 in lobular and ductal breast cancer. Proc Natl Acad Sci U S A 111:1933-8 (2014). PubMed: 24449868
    • Yang Z  et al. {beta}-Agonist-associated Reduction in RGS5 Expression Promotes Airway Smooth Muscle Hyper-responsiveness. J Biol Chem 286:11444-55 (2011). IHC-Fr . PubMed: 21278382
    • Muthusamy S  et al. Estrogen receptor ß and 17ß-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer. Proc Natl Acad Sci U S A 108:20090-4 (2011). IHC-P . PubMed: 22114194

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    GFP expresssing Mouse astrocytes in brain

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Formalin-fixed paraffin wax section was subjected to HIER before incubating in anti GFP ( ab13970). Section was then incubated with ab6876 ( 1/200) followed by stABCpx. GFP was visualised using DAB chromogen

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 25 2013

    Question

    I would like to know were is exacli attached the biotin. Is it only in the Fc region of the antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 16 2006

    Answer

    I have been informed that amine reactive biotins were used to conjugate Lysine sites of antibodies. It is not only in the Fc region. Please do not hesitate to contact us again if you need more information,

    Read More

    Abcam Scientific Support

    Answered on Jun 19 2006

    Question

    I am thinking of using this antibody as a negative control for a biotinylated goat polyclonal Ab. Have you got any data regarding cross-reactions with human, rabbit and mouse proteins (especially Ig)?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 16 2003

    Answer

    Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. At this time, we do not have any data about cross-reactivity with human, rabbit and mouse proteins.

    Read More

    Abcam Scientific Support

    Answered on Oct 16 2003

    Question

    Is this Antibody compartible (regarding sensivity) with Antirabbit IgG-Biotin from DAKO?

    Read More

    Abcam community

    Verified customer

    Asked on May 31 2001

    Answer

    We don't have any data on the sensitivity of antibodies from DAKO, so its' difficult to compare. We do know that this antibody recognises 1µg chicken IgG at a working dilution of at least 1/20000.

    Read More

    Abcam Scientific Support

    Answered on May 31 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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