Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
Key features and details
- Goat Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgY
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
Overview
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Product name
Goat Anti-Chicken IgY H&L (Biotin)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Tested applications
Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
Immunogen
chicken IgY whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/500 - 1/3000.
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| Dot blot |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent dilution.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| Immunomicroscopy |
Use at an assay dependent concentration.
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| WB |
1/3000 - 1/30000.
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| Notes |
|---|
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ICC/IF
1/500 - 1/3000. |
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Dot blot
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent dilution. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/3000 - 1/30000. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.
ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (6)
ab6876 has been referenced in 6 publications.
- Forbes D et al. Liquid Dermal Scaffold With Adipose-Derived Stem Cells Improve Tissue Quality in a Murine Model of Impaired Wound Healing. J Burn Care Res 40:550-557 (2019). PubMed: 31188436
- Wang L et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). PubMed: 29670081
- Huang B et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . PubMed: 27688768
- Huang B et al. Differential expression of estrogen receptor a, ß1, and ß2 in lobular and ductal breast cancer. Proc Natl Acad Sci U S A 111:1933-8 (2014). PubMed: 24449868
- Yang Z et al. {beta}-Agonist-associated Reduction in RGS5 Expression Promotes Airway Smooth Muscle Hyper-responsiveness. J Biol Chem 286:11444-55 (2011). IHC-Fr . PubMed: 21278382
- Muthusamy S et al. Estrogen receptor ß and 17ß-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer. Proc Natl Acad Sci U S A 108:20090-4 (2011). IHC-P . PubMed: 22114194
