Goat Anti-Chicken IgY H&L (Biotin) (ab97133)
Key features and details
- Goat Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IHC-P, ICC/IF, WB
Overview
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Product name
Goat Anti-Chicken IgY H&L (Biotin)
See all IgY secondary antibodies -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ELISA, IHC-P, ICC/IF, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/20000 - 1/400000. (Primary).
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IHC-P |
1/500 - 1/5000.
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ICC/IF |
1/200 - 1/1000.
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WB |
1/20000 - 1/400000. (Chemiluminescent).
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Notes |
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ELISA
1/20000 - 1/400000. (Primary). |
IHC-P
1/500 - 1/5000. |
ICC/IF
1/200 - 1/1000. |
WB
1/20000 - 1/400000. (Chemiluminescent). |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab97133 has been referenced in 1 publication.
- Pasini FS et al. A gene expression profile related to immune dampening in the tumor microenvironment is associated with poor prognosis in gastric adenocarcinoma. J Gastroenterol 49:1453-66 (2014). IHC . PubMed: 24217965