Overview

  • Product name

    Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed
    See all IgY secondary antibodies
  • Description

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Cy3 ®), pre-adsorbed
  • Host species

    Goat
  • Target species

    Chicken
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Chicken IgY.

  • Conjugation

    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer



As we discussed, please send me the images of the staining. I would suggest using a positive control and confirming the GFP transfection, but trying a fluorophore from an infrared channel where the auto-fluorescence is lower may be a good solution.

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Answer

Thank you for contacting us.

I have included with this email 2 documents about autofluorescence and tips for reducing this as requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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