Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)

Reviews (1)Q&A (1)References (30)
Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)

    Key features and details

    • Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 488)
    • Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: IHC-Fr, IHC-FoFr, Flow Cyt, ICC/IF, IHC-P

    Conjugates logo Related conjugates and formulations

    Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 550 DyLight® 594 FITC Gold 10nm Gold 25nm HRP HRP PE Texas Red ® Unconjugated Unconjugated

    Overview

    • Product name

      Goat Anti-Chicken IgY H&L (DyLight® 488)
      See all IgY secondary antibodies

    • Description

      Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 488)

    • Host species

      Goat

    • Target species

      Chicken

    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    • Tested applications

      Suitable for: IHC-Fr, IHC-FoFr, Flow Cyt, ICC/IF, IHC-Pmore details

    • Conjugation

      DyLight® 488. Ex: 493nm, Em: 518nm

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab96947 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 24284943
    IHC-FoFr (1)
    Use at an assay dependent concentration. PubMed: 24510125
    Flow Cyt
    1/50 - 1/200.
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.
    Notes
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 24284943
    IHC-FoFr
    Use at an assay dependent concentration. PubMed: 24510125
    Flow Cyt
    1/50 - 1/200.
    ICC/IF
    1/50 - 1/500.
    IHC-P
    1/50 - 1/500.

    Images

    • Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)
      Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (30)

    Publishing research using ab96947? Please let us know so that we can cite the reference in this datasheet.

    ab96947 has been referenced in 30 publications.

    • Barbier M  et al. Projections from the dorsomedial division of the bed nucleus of the stria terminalis to hypothalamic nuclei in the mouse. J Comp Neurol 529:929-956 (2021). PubMed: 32678476
    • Bandarabadi M  et al. A role for spindles in the onset of rapid eye movement sleep. Nat Commun 11:5247 (2020). PubMed: 33067436
    • Facchin L  et al. Slow Waves Promote Sleep-Dependent Plasticity and Functional Recovery after Stroke. J Neurosci 40:8637-8651 (2020). PubMed: 33087472
    • Wu X  et al. CARD3 Promotes Cerebral Ischemia-Reperfusion Injury Via Activation of TAK1. J Am Heart Assoc 9:e014920 (2020). PubMed: 32349637
    • Li Y  et al. Branching points of primary afferent fibers are vital for the modulation of fiber excitability by epidural DC polarization and by GABA in the rat spinal cord. J Neurophysiol 124:49-62 (2020). PubMed: 32459560
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Excellent detection of chicken IgY

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Mouse received brain injury and the brain was formalin-fixed and frozen. GFAP was stained with chicken anti-GFAP antibody. This Goat anti-Chicken was diluted 1:500 and it developed a strong signal with the primary. The tissue was also counterstained with DAPI (blue).

    Abcam user community

    Verified customer

    Submitted Dec 03 2020

    Question

    Hi, I am looking for an antibody with could works with Anti-lamin antibody (ab14309) for immunostaining, I found ab96951 and ab96947 might be the right secondary antibodies. However, I am confused by the isotype IgY or IgG in datasheet. Could you please suggest me which one should be chosen and why? Thank you very much Secton

    Read More
    Answer

    Thank you for contacting us.

    Chicken, like all birds, produce a different isotype of immunoglobins, the IgY. The secondary antibodies are derived in goat which is a mammal, and the most common isotype for commercial availible antibodies here is IgG.
    The goat IgG will bind the chicken IgY, because the IgY was the immunogen.

    The two secondary antibodies differ in their purification grade: ab96951 is pre-absorbed, which means that cross reactivity with other species are less likely. This is important if you perfom double staing, or using the serum of a different species for blocking. If you are not going to do this, the ab96947should be fine for you as well.



    I have checked the ab14309 datasheet, and it looks like the antibody hasn't been tested on the endogenous protein yet.Therefore, I can offer a discount off a future purchase if you buy ab14309 now, test it in endogenous Lamin A and submit feedback to us in the form of an Abreview. It doesn' t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.


    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab14309 in endogenous Lamin A. I will then send a discount code. This code must be issued before purchasing ab14309 so please wait for my reply before ordering.
    2. Purchase ab14309 either by phone, fax, or online (www.abcam.com).
    3. Test iton the endogenous Lamin A in immunostaining.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.


    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab14309 turns out to be unsuitable for endogenous Lamin A, you will still receive the discount on your next purchase after your Abreview has been submitted.


    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

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