Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)

Submit a review Q&A (1)References (19)

Overview

  • Product name

    Goat Anti-Chicken IgY H&L (DyLight® 488)
    See all IgY secondary antibodies

  • Description

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 488)

  • Host species

    Goat

  • Target species

    Chicken

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: IHC-Fr, IHC-FoFr, Flow Cyt, ICC/IF, IHC-Pmore details

  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24284943
IHC-FoFr Use at an assay dependent concentration. PubMed: 24510125
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)
    Goat Anti-Chicken IgY H&L (DyLight® 488) (ab96947)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

See all 19 Publications for this product

Publishing research using ab96947? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Hi, I am looking for an antibody with could works with Anti-lamin antibody (ab14309) for immunostaining, I found ab96951 and ab96947 might be the right secondary antibodies. However, I am confused by the isotype IgY or IgG in datasheet. Could you please suggest me which one should be chosen and why? Thank you very much Secton

Read More
Answer

Thank you for contacting us.

Chicken, like all birds, produce a different isotype of immunoglobins, the IgY. The secondary antibodies are derived in goat which is a mammal, and the most common isotype for commercial availible antibodies here is IgG.
The goat IgG will bind the chicken IgY, because the IgY was the immunogen.

The two secondary antibodies differ in their purification grade: ab96951 is pre-absorbed, which means that cross reactivity with other species are less likely. This is important if you perfom double staing, or using the serum of a different species for blocking. If you are not going to do this, the ab96947should be fine for you as well.



I have checked the ab14309 datasheet, and it looks like the antibody hasn't been tested on the endogenous protein yet.Therefore, I can offer a discount off a future purchase if you buy ab14309 now, test it in endogenous Lamin A and submit feedback to us in the form of an Abreview. It doesn' t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab14309 in endogenous Lamin A. I will then send a discount code. This code must be issued before purchasing ab14309 so please wait for my reply before ordering.
2. Purchase ab14309 either by phone, fax, or online (www.abcam.com).
3. Test iton the endogenous Lamin A in immunostaining.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.


We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab14309 turns out to be unsuitable for endogenous Lamin A, you will still receive the discount on your next purchase after your Abreview has been submitted.


Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com