Key features and details
- Goat Anti-Chicken IgY H&L (DyLight® 550)
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Product nameGoat Anti-Chicken IgY H&L (DyLight® 550)
See all IgY secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-Pmore details
ConjugationDyLight® 550. Ex: 562nm, Em: 576nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
General notesDyLight® 550 replaces DyLight® 549.
Our Abpromise guarantee covers the use of ab96948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/200.|
|ICC/IF||1/50 - 1/500.|
|IHC-P||1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab96948 has been referenced in 1 publication.
- Ghidinelli M et al. Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination. PLoS Biol 15:e2001408 (2017). IHC ; Mouse . PubMed: 28636612