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    goat-chicken-igy-hl-dylight-550-ab96948.pdf

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Secondary antibodies anti-Chicken IgY Fluorophore DyLight® 550
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Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)

  • Datasheet
Submit a review Q&A (1)References (1)

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Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)

    Key features and details

    • Goat Anti-Chicken IgY H&L (DyLight® 550)
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: Flow Cyt, ICC/IF, IHC-P

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    Overview

    • Product name

      Goat Anti-Chicken IgY H&L (DyLight® 550)
      See all IgY secondary antibodies
    • Host species

      Goat
    • Target species

      Chicken
    • Specificity

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
    • Tested applications

      Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
    • Conjugation

      DyLight® 550. Ex: 562nm, Em: 576nm

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • General notes

      DyLight® 550 replaces DyLight® 549.
    • Research areas

      • Secondary antibodies
      • anti-Chicken
      • IgY
      • Fluorophore
      • DyLight® 550

    Associated products

      Applications

      Our Abpromise guarantee covers the use of ab96948 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      Flow Cyt 1/50 - 1/200.
      ICC/IF 1/50 - 1/500.
      IHC-P 1/50 - 1/500.

      DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

      Images

      • Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)
        Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)
        Emission spectra of DyLight® fluorochromes available in our catalog.
        Line colors represent the approximate visible colors of the wavelength maxima.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab96948? Please let us know so that we can cite the reference in this datasheet.

      ab96948 has been referenced in 1 publication.

      • Ghidinelli M  et al. Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination. PLoS Biol 15:e2001408 (2017). IHC ; Mouse . PubMed: 28636612

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      What is the ratio of Dylight molecule conjugate to antibody? Where Dylight is attached to the antibody?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 24 2011

      Answer

      Thank you for contacting us. Approximately 4-6 Dylight molecules gets attached to a antibody. The Dylight fluorophore is conjugated at Fc region of the antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Aug 24 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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