Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)

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Overview

  • Product name

    Goat Anti-Chicken IgY H&L (DyLight® 550)
    See all IgY secondary antibodies

  • Host species

    Goat

  • Target species

    Chicken

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    DyLight® 550 replaces DyLight® 549.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.

Images

  • Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)
    Goat Anti-Chicken IgY H&L (DyLight® 550) (ab96948)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:

  • Ghidinelli M  et al. Laminin 211 inhibits protein kinase A in Schwann cells to modulate neuregulin 1 type III-driven myelination. PLoS Biol 15:e2001408 (2017). IHC ; Mouse . Read more (PubMed: 28636612) »
See 1 Publication for this product

Publishing research using ab96948? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

What is the ratio of Dylight molecule conjugate to antibody? Where Dylight is attached to the antibody?

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Answer

Thank you for contacting us. Approximately 4-6 Dylight molecules gets attached to a antibody. The Dylight fluorophore is conjugated at Fc region of the antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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