Goat Anti-Chicken IgY H&L (DyLight® 650) preadsorbed (ab96954)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product name
    Goat Anti-Chicken IgY H&L (DyLight® 650) preadsorbed
    See all IgY secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed
  • Host species
    Goat
  • Target species
    Chicken
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat more details
  • Conjugation
    DyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 650 replaces DyLight® 649.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • DyLight®-Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed(ab96954)
    DyLight®-Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed(ab96954)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

This product has been referenced in:
  • Asselin-Mullen P  et al. Protein interaction network of alternatively spliced NudCD1 isoforms. Sci Rep 7:12987 (2017). Read more (PubMed: 29021621) »
  • Okamoto M  et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P . Read more (PubMed: 24278321) »

See all 2 Publications for this product

Publishing research using ab96954? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

How many DyLight 650 fluorophore molecules are conjugated to each IgG molecule?

Thank you for your inquiry.


I heard back from the lab that the ratio of DyLight650 to Protein (F/P)varies per lot for ab96954.

Lot GR66644-3: F/P = 5.5

I hope this information helps. Please let me know if you need ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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