Goat Anti-Chicken IgY H&L (DyLight® 650) preadsorbed (ab96954)
Key features and details
- Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Overview
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Product name
Goat Anti-Chicken IgY H&L (DyLight® 650) preadsorbed
See all IgY secondary antibodies -
Description
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650), pre-adsorbed -
Host species
Goat -
Target species
Chicken -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Minimal
cross-reactivity
Cow, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Associated products
Applications
Our Abpromise guarantee covers the use of ab96954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
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| ICC/IF | 1/50 - 1/500. | |
| Flow Cyt | 1/50 - 1/200. |
Images
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Goat Anti-Chicken IgY H&L (DyLight® 650) preadsorbed (ab96954)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (3)
ab96954 has been referenced in 3 publications.
- von Twickel A et al. Individual Dopaminergic Neurons of Lamprey SNc/VTA Project to Both the Striatum and Optic Tectum but Restrict Co-release of Glutamate to Striatum Only. Curr Biol 29:677-685.e6 (2019). PubMed: 30713108
- Asselin-Mullen P et al. Protein interaction network of alternatively spliced NudCD1 isoforms. Sci Rep 7:12987 (2017). PubMed: 29021621
- Okamoto M et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P . PubMed: 24278321
